BBa_C0061 1 luxI autoinducer synthetase for AHL 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z <em>V. fischeri</em> <genbank>AF170104</genbank> Released HQ 2013 Synthesizes 3OC₆HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p> false false _1_ 0 24 7 In stock false <P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr annotation7038 1 BBa_C0061 range7038 1 1 618 annotation1761 1 luxI range1761 1 1 579 annotation2213985 1 Help:Barcodes range2213985 1 619 643 annotation1760 1 LVA range1760 1 580 611 BBa_K258004 1 BBa_K258004 Keratinocyte growth factor (KGF) 2009-10-03T11:00:00Z 2015-05-08T01:11:42Z Gene was synthesized at GENEART. Keratinocyte growth factor (KGF) is a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial tissues.Use of recombinant human KGF (palifermin) in patients with hematologic malignancies reduces the incidence and duration of severe oral mucositis experienced after intensive chemoradiotherapy. These results suggest that KGF may be useful in the treatment of patients with other kinds of tumors, including those of epithelial origin.[1]The potential effect of KGF on wound healing was assessed in vitro by measuring randomized migration and plasminogen activator (PA) activity of keratinocytes in response to the growth factor. Incubation of normal human keratinocytes with KGF in modified MCDB 153 medium significantly stimulated cell migration[2] The Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium.[3] . KGF is weakly expressed in human skin, but is strongly upregulated in dermal fibroblasts after skin injury. Its binding to a transmembrane receptor on keratinocytes induces proliferation and migration of these cells. Furthermore, KGF has been shown to protect epithelial cells from the toxic effects of reactive oxygen species. We have identified a series of KGF-regulated genes that are likely to play a role in these processes. In addition to KGF, activin seems to be a novel player in wound healing.[4] false false _358_ 0 4260 9 It's complicated true The gene is in pMA vector having biobrick restriction sites(Ecor1, Xba1 and Spe1,Pst1,respectively) false Ozkan IS BBa_K082022 1 BBa_K082022 B0034-C0051-B0015 2008-10-28T12:00:00Z 2015-05-08T01:08:35Z none Released HQ 2013 none false false _228_ 0 1560 9 In stock true none false Yanting Xue , He Shen component2249416 1 BBa_B0034 component2249420 1 BBa_C0051 component2249427 1 BBa_B0015 annotation2249420 1 BBa_C0051 range2249420 1 19 793 annotation2249427 1 BBa_B0015 range2249427 1 802 930 annotation2249416 1 BBa_B0034 range2249416 1 1 12 BBa_K235015 1 BBa_K235015 [R0051][B0034] (pLambda+RBS) 2009-09-02T11:00:00Z 2015-05-08T01:11:35Z R0051+B0034 Just an intermediate part. false true _336_ 0 5167 9 It's complicated false Constructed using three-antibiotic assembly. On plasmid pSB1C3. false Kyliah Clarkson component2018444 1 BBa_B0034 component2018439 1 BBa_R0051 annotation2018439 1 BBa_R0051 range2018439 1 1 49 annotation2018444 1 BBa_B0034 range2018444 1 58 69 BBa_R0051 1 cI lam promoter (lambda cI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false true _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2024 1 OR1 range2024 1 25 41 annotation2022 1 -10 range2022 1 38 43 annotation2023 1 -35 range2023 1 15 20 annotation2025 1 OR2 range2025 1 1 17 annotation7067 1 BBa_R0051 range7067 1 1 49 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I751300 1 BBa_I751300 pBR322 luxI 2007-10-22T11:00:00Z 2015-08-31T04:08:06Z Released HQ 2013 -RBS-luxI- on pBR322 false false _151_ 0 2188 9 In stock false false Kenichiro Iwasaki component2243932 1 BBa_C0261 annotation2243932 1 BBa_C0261 range2243932 1 1 661 BBa_K258006 1 BBa_K258006 Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1 2009-10-08T11:00:00Z 2015-05-08T01:11:42Z The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter. TliA has four glycinerich repeats (GGXGXD) in its C-terminus, which appear in many ABC transporter-secreted proteins.Export of fusion proteins with the whole TliA through the ABC transporter was evident on the basis of lipase enzymatic activity. Upon supplementation of E. coli with ABC transporter, EGF-TliA was excreted into the culture supernatant.Whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF which has 3 disulfide bonds in E. coli supplemented with ABC transporter. Activity domain (residues 1???268) and secretion/chaperon domain (residues 279???476). In our experiment, we observed that TliA fused proteins were excreted to supernatant culture succesfully by detecting lipase activity with tributyrin and spectrophotometric detection with the substrate p-nitrophenyl phosphate at 420 nm. At the experiments, Tlia fused proteins were excreted ten-fold more with ABC transporter PrtDEF of Erwinia chrysanthemi. false false _358_ 0 4260 9 Not in stock true The sequence was optimized for E.coli with Biobrick restriction sites false Jung Hoon Ahn from Korea Advanced Institute of Science and Technology BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K094130 1 BBa_K094130 Rbs-mRFP1-terminator 2008-10-12T11:00:00Z 2015-05-08T01:08:40Z Adapted from BBa_J04450 Released HQ 2013 This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit. false false _255_ 0 2589 9 In stock false no special attentions false Shi Lei component2249228 1 BBa_E1010 component2249231 1 BBa_B0012 component2249225 1 BBa_B0034 component2249229 1 BBa_B0010 annotation2249229 1 BBa_B0010 range2249229 1 733 812 annotation2249228 1 BBa_E1010 range2249228 1 19 724 annotation2249231 1 BBa_B0012 range2249231 1 821 861 annotation2249225 1 BBa_B0034 range2249225 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_C0261 1 BBa_C0261 AHL-making Enzyme, luxI (+RBS) 2004-08-22T11:00:00Z 2015-08-31T04:07:24Z Released HQ 2013 B0034.C0061 false false _6_ 0 159 7 In stock true true robertb component1060413 1 BBa_C0061 component1060403 1 BBa_B0034 annotation1060403 1 BBa_B0034 range1060403 1 1 12 annotation1060413 1 BBa_C0061 range1060413 1 19 636 BBa_C0051 1 cI lam cI repressor from E. coli phage lambda (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Released HQ 2013 Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P> true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation23334 1 cI lambda range23334 1 4 711 annotation23335 1 LVA range23335 1 712 744 annotation2213991 1 Help:Barcodes range2213991 1 751 775 BBa_K258012 1 BBa_K258012 AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z This project has been designing synthetically we wanted to be synthesized by bacteria, is KGF(Keratinocyte Growth Factor).The protein composed of 194 aminoacids and has a function to renew epitel tissue in healing period. However, it is not observed to cause any effect on cell differentiaiton.It speeds up the healing process. This is the reason why KGF gene is also important for our project. E.Coli has a spesific protein to communicate among them. This protein is called AL-2. And, the concentration of AL-2 just depends on bacteria concentration. For our mechanism, when EGF synthesizing bacteria has reached certain amount, AL-2 protein will start to be produced.In that part, our aim is to make AL-2 as a kind of signal and with the help of that signal, to start the KGF synthesis.As a result, when one type of bacteria produce EGF, the other types produce KGF. But, it should be noted that after that process, there can be a danger: the huge and uncontrolled amount of EGF and KGF. To avoid that danger, we developed another quorum sensing mechanism. As a result, all two mechanisms are completed with lysis process. false false _358_ 0 4260 9 Not in stock true our part has been bulding by biobrirck assembling technique false Cihan Tastan and Ozkan IS component2310587 1 BBa_K131017 component2310601 1 BBa_K082022 component2310631 1 BBa_K094130 component2310611 1 BBa_K258006 component2310609 1 BBa_K235015 component2310610 1 BBa_K258004 component2310619 1 BBa_I751300 annotation2310601 1 BBa_K082022 range2310601 1 284 1213 annotation2310587 1 BBa_K131017 range2310587 1 1 275 annotation2310619 1 BBa_I751300 range2310619 1 3231 3891 annotation2310610 1 BBa_K258004 range2310610 1 1297 1788 annotation2310611 1 BBa_K258006 range2310611 1 1795 3222 annotation2310631 1 BBa_K094130 range2310631 1 3900 4760 annotation2310609 1 BBa_K235015 range2310609 1 1222 1290 BBa_K131017 1 p_qrr4 p_qrr4 from <I>Vibrio harveyi</I> 2008-10-27T12:00:00Z 2015-05-08T01:09:47Z >gi|156527546:c1833759-1833376 Vibrio harveyi ATCC BAA-1116 chromosome II, complete sequence qrr4 promoter from Vibrio harveyi false true _259_ 0 1653 9 In stock true 5' upstream sequence of qrr4 containing -12 and -24 regions (specific for Sigma factor 54) true Sonja Georgijevic annotation1990440 1 -12 region range1990440 1 262 264 annotation1990436 1 -24 region range1990436 1 250 254 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_R0051_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_K094130_sequence 1 aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K131017_sequence 1 gtatcagcaaaaacactacggtggataatcagtaaaaccatgaaactagagccccgcacacttgcggggctttttaattttgaatttctttcttattaaaacgccatttttctgataaatgtattagtagcaatgcgcatggtggcatatttgcatcattttgcattttgcaaatgcgatttgcaaaatgcgtgctcaataaagcaccaatatgcatcaggatcgaagaaaaaaggcgtttttaaaagttggcacgcatcgtgctttatacagat BBa_C0261_sequence 1 aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I751300_sequence 1 aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_C0061_sequence 1 atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_K258012_sequence 1 gtatcagcaaaaacactacggtggataatcagtaaaaccatgaaactagagccccgcacacttgcggggctttttaattttgaatttctttcttattaaaacgccatttttctgataaatgtattagtagcaatgcgcatggtggcatatttgcatcattttgcattttgcaaatgcgatttgcaaaatgcgtgctcaataaagcaccaatatgcatcaggatcgaagaaaaaaggcgtttttaaaagttggcacgcatcgtgctttatacagattactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgtgtaacgacatgacccctgagcaaatggcaacaaacgtgaactgtagtagtcctgaacgccacactcgctcttatgattatatggagggcggggatattcgtgttcgtcgcctgttctgccgtactcaatggtatctgcgcatcgataaacgtggtaaagtgaaaggcacccaagaaatgaaaaacaactataacatcatggaaatccgtaccgtggcagttggtattgtagcgatcaaaggcgtggaatccgagttctatctggcaatgaacaaagagggcaaactgtatgccaaaaaagagtgtaacgaggactgtaacttcaaagaactgatcctggagaaccattataacacctatgccagcgccaaatggacccataacggtggtgaaatgttcgtggccctgaaccaaaaaggcattccggtccgtggcaaaaaaacgaaaaaagagcagaaaaccgcccacttcctgcctatggccattacatactagatgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctgggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtggaacgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccgtgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgcgctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggtaatgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccggccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttccagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcagactctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttcttactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0051_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc BBa_K258004_sequence 1 atgtgtaacgacatgacccctgagcaaatggcaacaaacgtgaactgtagtagtcctgaacgccacactcgctcttatgattatatggagggcggggatattcgtgttcgtcgcctgttctgccgtactcaatggtatctgcgcatcgataaacgtggtaaagtgaaaggcacccaagaaatgaaaaacaactataacatcatggaaatccgtaccgtggcagttggtattgtagcgatcaaaggcgtggaatccgagttctatctggcaatgaacaaagagggcaaactgtatgccaaaaaagagtgtaacgaggactgtaacttcaaagaactgatcctggagaaccattataacacctatgccagcgccaaatggacccataacggtggtgaaatgttcgtggccctgaaccaaaaaggcattccggtccgtggcaaaaaaacgaaaaaagagcagaaaaccgcccacttcctgcctatggccattaca BBa_K258006_sequence 1 atgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctgggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtggaacgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccgtgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgcgctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggtaatgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccggccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttccagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcagactctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttct BBa_K082022_sequence 1 aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K235015_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z