BBa_K258012
type: ComponentDefinition
persistent identity: BBa K258012
display id: BBa_K258012
version: 1
was generated by: iGEM to SBOL conversion
description: AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: This project has been designing synthetically
owned by: james
owned by: myers
mutable description: we wanted to be synthesized by bacteria, is KGF(Keratinocyte Growth Factor).The protein composed of 194 aminoacids and has a function to renew epitel tissue in healing period. However, it is not observed to cause any effect on cell differentiaiton.It speeds up the healing process. This is the reason why KGF gene is also important for our project. E.Coli has a spesific protein to communicate among them. This protein is called AL-2. And, the concentration of AL-2 just depends on bacteria concentration. For our mechanism, when EGF synthesizing bacteria has reached certain amount, AL-2 protein will start to be produced.In that part, our aim is to make AL-2 as a kind of signal and with the help of that signal, to start the KGF synthesis.As a result, when one type of bacteria produce EGF, the other types produce KGF. But, it should be noted that after that process, there can be a danger: the huge and uncontrolled amount of EGF and KGF. To avoid that danger, we developed another quorum sensing mechanism. As a result, all two mechanisms are completed with lysis process.
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: our part has been bulding by biobrirck assembling technique
star: false
creator: Cihan Tastan and Ozkan IS
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K094130
component: BBa_I751300
component: BBa_K258006
component: BBa_K258004
component: BBa_K235015
component: BBa_K082022
component: BBa_K131017
sequence annotation: BBa_K131017
sequence annotation: BBa_K082022
sequence annotation: BBa_K235015
sequence annotation: BBa_K258004
sequence annotation: BBa_K258006
sequence annotation: BBa_I751300
sequence annotation: BBa_K094130
sequence: https://synbiohub.org/public/igem/BBa_K258012_sequence/1

BBa_K094130
type: Component
persistent identity: component2310631
display id: component2310631
version: 1
top level: BBa_K258012
definition: BBa_K094130
access: public

BBa_I751300
type: Component
persistent identity: component2310619
display id: component2310619
version: 1
top level: BBa_K258012
definition: BBa_I751300
access: public

BBa_K258006
type: Component
persistent identity: component2310611
display id: component2310611
version: 1
top level: BBa_K258012
definition: BBa_K258006
access: public

BBa_K258004
type: Component
persistent identity: component2310610
display id: component2310610
version: 1
top level: BBa_K258012
definition: BBa_K258004
access: public

BBa_K235015
type: Component
persistent identity: component2310609
display id: component2310609
version: 1
top level: BBa_K258012
definition: BBa_K235015
access: public

BBa_K082022
type: Component
persistent identity: component2310601
display id: component2310601
version: 1
top level: BBa_K258012
definition: BBa_K082022
access: public

BBa_K131017
type: Component
persistent identity: component2310587
display id: component2310587
version: 1
top level: BBa_K258012
definition: p_qrr4
access: public

BBa_K131017
type: SequenceAnnotation
persistent identity: annotation2310587
display id: annotation2310587
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310587/range2310587/1
component: BBa_K131017

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type: Range
persistent identity: range2310587
display id: range2310587
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 1
end: 275
orientation: inline

BBa_K082022
type: SequenceAnnotation
persistent identity: annotation2310601
display id: annotation2310601
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310601/range2310601/1
component: BBa_K082022

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type: Range
persistent identity: range2310601
display id: range2310601
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 284
end: 1213
orientation: inline

BBa_K235015
type: SequenceAnnotation
persistent identity: annotation2310609
display id: annotation2310609
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310609/range2310609/1
component: BBa_K235015

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type: Range
persistent identity: range2310609
display id: range2310609
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 1222
end: 1290
orientation: inline

BBa_K258004
type: SequenceAnnotation
persistent identity: annotation2310610
display id: annotation2310610
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310610/range2310610/1
component: BBa_K258004

https://synbiohub.org/public/igem/BBa_K258012/annotation2310610/range2310610/1
type: Range
persistent identity: range2310610
display id: range2310610
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 1297
end: 1788
orientation: inline

BBa_K258006
type: SequenceAnnotation
persistent identity: annotation2310611
display id: annotation2310611
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310611/range2310611/1
component: BBa_K258006

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type: Range
persistent identity: range2310611
display id: range2310611
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 1795
end: 3222
orientation: inline

BBa_I751300
type: SequenceAnnotation
persistent identity: annotation2310619
display id: annotation2310619
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310619/range2310619/1
component: BBa_I751300

https://synbiohub.org/public/igem/BBa_K258012/annotation2310619/range2310619/1
type: Range
persistent identity: range2310619
display id: range2310619
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 3231
end: 3891
orientation: inline

BBa_K094130
type: SequenceAnnotation
persistent identity: annotation2310631
display id: annotation2310631
version: 1
top level: BBa_K258012
location: https://synbiohub.org/public/igem/BBa_K258012/annotation2310631/range2310631/1
component: BBa_K094130

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type: Range
persistent identity: range2310631
display id: range2310631
version: 1
top level: BBa_K258012
direction: direction/reverse
start: 3900
end: 4760
orientation: inline

BBa_I751300
type: ComponentDefinition
persistent identity: BBa I751300
display id: BBa_I751300
version: 1
was generated by: iGEM to SBOL conversion
description: pBR322 luxI
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -RBS-luxI- on pBR322
discontinued: false
dominant: false
experience: experience/None
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0261
sequence annotation: BBa_C0261
sequence: https://synbiohub.org/public/igem/BBa_I751300_sequence/1

BBa_C0261
type: Component
persistent identity: component2243932
display id: component2243932
version: 1
top level: BBa_I751300
definition: BBa_C0261
access: public

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2243932
display id: annotation2243932
version: 1
top level: BBa_I751300
location: https://synbiohub.org/public/igem/BBa_I751300/annotation2243932/range2243932/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_I751300/annotation2243932/range2243932/1
type: Range
persistent identity: range2243932
display id: range2243932
version: 1
top level: BBa_I751300
direction: direction/reverse
start: 1
end: 661
orientation: inline

BBa_K094130
type: ComponentDefinition
persistent identity: BBa K094130
display id: BBa_K094130
version: 1
was generated by: iGEM to SBOL conversion
description: Rbs-mRFP1-terminator
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: Adapted from BBa_J04450
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special attentions
star: false
creator: Shi Lei
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K094130_sequence/1

BBa_B0034
type: Component
persistent identity: component2249225
display id: component2249225
version: 1
top level: BBa_K094130
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component2249229
display id: component2249229
version: 1
top level: BBa_K094130
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component2249228
display id: component2249228
version: 1
top level: BBa_K094130
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component2249231
display id: component2249231
version: 1
top level: BBa_K094130
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249225
display id: annotation2249225
version: 1
top level: BBa_K094130
location: https://synbiohub.org/public/igem/BBa_K094130/annotation2249225/range2249225/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094130/annotation2249225/range2249225/1
type: Range
persistent identity: range2249225
display id: range2249225
version: 1
top level: BBa_K094130
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2249228
display id: annotation2249228
version: 1
top level: BBa_K094130
location: https://synbiohub.org/public/igem/BBa_K094130/annotation2249228/range2249228/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K094130/annotation2249228/range2249228/1
type: Range
persistent identity: range2249228
display id: range2249228
version: 1
top level: BBa_K094130
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2249229
display id: annotation2249229
version: 1
top level: BBa_K094130
location: https://synbiohub.org/public/igem/BBa_K094130/annotation2249229/range2249229/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K094130/annotation2249229/range2249229/1
type: Range
persistent identity: range2249229
display id: range2249229
version: 1
top level: BBa_K094130
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2249231
display id: annotation2249231
version: 1
top level: BBa_K094130
location: https://synbiohub.org/public/igem/BBa_K094130/annotation2249231/range2249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K094130/annotation2249231/range2249231/1
type: Range
persistent identity: range2249231
display id: range2249231
version: 1
top level: BBa_K094130
direction: direction/forward
start: 821
end: 861
orientation: inline

BBa_K258006
type: ComponentDefinition
persistent identity: BBa K258006
display id: BBa_K258006
version: 1
was generated by: iGEM to SBOL conversion
description: Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1
created: 2009-10-08T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter.
owned by: james
owned by: myers
mutable description: TliA has four glycinerich repeats (GGXGXD) in its C-terminus, which appear in many ABC transporter-secreted proteins.Export of fusion proteins with the whole TliA through the ABC transporter was evident on the basis of lipase enzymatic activity. Upon supplementation of E. coli with ABC transporter, EGF-TliA was excreted into the culture supernatant.Whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF which has 3 disulfide bonds in E. coli supplemented with ABC transporter. Activity domain (residues 1???268) and secretion/chaperon domain (residues 279???476). In our experiment, we observed that TliA fused proteins were excreted to supernatant culture succesfully by detecting lipase activity with tributyrin and spectrophotometric detection with the substrate p-nitrophenyl phosphate at 420 nm. At the experiments, Tlia fused proteins were excreted ten-fold more with ABC transporter PrtDEF of Erwinia chrysanthemi.
discontinued: false
dominant: false
experience: experience/Works
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The sequence was optimized for E.coli with Biobrick restriction sites
star: false
creator: Jung Hoon Ahn from Korea Advanced Institute of Science and Technology
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K258006_sequence/1

BBa_K258004
type: ComponentDefinition
persistent identity: BBa K258004
display id: BBa_K258004
version: 1
was generated by: iGEM to SBOL conversion
description: Keratinocyte growth factor (KGF)
created: 2009-10-03T11:00:00Z
modified: 2015-05-08T01:11:42Z
mutable provenance: Gene was synthesized at GENEART.
owned by: james
owned by: myers
mutable description: Keratinocyte growth factor (KGF) is a locally acting epithelial mitogen that is produced by cells of mesenchymal origin and has an important role in protecting and repairing epithelial tissues.Use of recombinant human KGF (palifermin) in patients with hematologic malignancies reduces the incidence and duration of severe oral mucositis experienced after intensive chemoradiotherapy. These results suggest that KGF may be useful in the treatment of patients with other kinds of tumors, including those of epithelial origin.[1]The potential effect of KGF on wound healing was assessed in vitro by measuring randomized migration and plasminogen activator (PA) activity of keratinocytes in response to the growth factor. Incubation of normal human keratinocytes with KGF in modified MCDB 153 medium significantly stimulated cell migration[2] The Keratinocyte Growth Factor (KGF), also known as FGF7, is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium.[3] . KGF is weakly expressed in human skin, but is strongly upregulated in dermal fibroblasts after skin injury. Its binding to a transmembrane receptor on keratinocytes induces proliferation and migration of these cells. Furthermore, KGF has been shown to protect epithelial cells from the toxic effects of reactive oxygen species. We have identified a series of KGF-regulated genes that are likely to play a role in these processes. In addition to KGF, activin seems to be a novel player in wound healing.[4]
discontinued: false
dominant: false
experience: experience/None
group u list: _358_
m user id: 0
owner id: 4260
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The gene is in pMA vector having biobrick restriction sites(Ecor1, Xba1 and Spe1,Pst1,respectively)
star: false
creator: Ozkan IS
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K258004_sequence/1

p_qrr4
type: ComponentDefinition
persistent identity: BBa K131017
display id: BBa_K131017
version: 1
was generated by: iGEM to SBOL conversion
description: p_qrr4 from Vibrio harveyi
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:47Z
mutable provenance: >gi|156527546:c1833759-1833376 Vibrio harveyi ATCC BAA-1116 chromosome II, complete sequence
owned by: james
owned by: myers
mutable description: qrr4 promoter from Vibrio harveyi
discontinued: false
dominant: true
experience: experience/None
group u list: _259_
m user id: 0
owner id: 1653
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: 5' upstream sequence of qrr4 containing -12 and -24 regions (specific for Sigma factor 54)
star: true
creator: Sonja Georgijevic
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -24 region
sequence annotation: -12 region
sequence: https://synbiohub.org/public/igem/BBa_K131017_sequence/1

-24 region
type: SequenceAnnotation
persistent identity: annotation1990436
display id: annotation1990436
version: 1
top level: p_qrr4
location: https://synbiohub.org/public/igem/BBa_K131017/annotation1990436/range1990436/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K131017/annotation1990436/range1990436/1
type: Range
persistent identity: range1990436
display id: range1990436
version: 1
top level: p_qrr4
direction: direction/forward
start: 250
end: 254
orientation: inline

-12 region
type: SequenceAnnotation
persistent identity: annotation1990440
display id: annotation1990440
version: 1
top level: p_qrr4
location: https://synbiohub.org/public/igem/BBa_K131017/annotation1990440/range1990440/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K131017/annotation1990440/range1990440/1
type: Range
persistent identity: range1990440
display id: range1990440
version: 1
top level: p_qrr4
direction: direction/forward
start: 262
end: 264
orientation: inline

BBa_K082022
type: ComponentDefinition
persistent identity: BBa K082022
display id: BBa_K082022
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-C0051-B0015
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: none
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: none
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: none
star: false
creator: Yanting Xue , He Shen
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0015
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K082022_sequence/1

BBa_B0015
type: Component
persistent identity: component2249427
display id: component2249427
version: 1
top level: BBa_K082022
definition: BBa_B0015
access: public

BBa_C0051
type: Component
persistent identity: component2249420
display id: component2249420
version: 1
top level: BBa_K082022
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component2249416
display id: component2249416
version: 1
top level: BBa_K082022
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249416
display id: annotation2249416
version: 1
top level: BBa_K082022
location: https://synbiohub.org/public/igem/BBa_K082022/annotation2249416/range2249416/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082022/annotation2249416/range2249416/1
type: Range
persistent identity: range2249416
display id: range2249416
version: 1
top level: BBa_K082022
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2249420
display id: annotation2249420
version: 1
top level: BBa_K082022
location: https://synbiohub.org/public/igem/BBa_K082022/annotation2249420/range2249420/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K082022/annotation2249420/range2249420/1
type: Range
persistent identity: range2249420
display id: range2249420
version: 1
top level: BBa_K082022
direction: direction/reverse
start: 19
end: 793
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249427
display id: annotation2249427
version: 1
top level: BBa_K082022
location: https://synbiohub.org/public/igem/BBa_K082022/annotation2249427/range2249427/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K082022/annotation2249427/range2249427/1
type: Range
persistent identity: range2249427
display id: range2249427
version: 1
top level: BBa_K082022
direction: direction/reverse
start: 802
end: 930
orientation: inline

BBa_K235015
type: ComponentDefinition
persistent identity: BBa K235015
display id: BBa_K235015
version: 1
was generated by: iGEM to SBOL conversion
description: [R0051][B0034] (pLambda+RBS)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: R0051+B0034
owned by: james
owned by: myers
mutable description: Just an intermediate part.
discontinued: false
dominant: true
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1C3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0051
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K235015_sequence/1

BBa_B0034
type: Component
persistent identity: component2018444
display id: component2018444
version: 1
top level: BBa_K235015
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2018439
display id: component2018439
version: 1
top level: BBa_K235015
definition: cI lam
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2018439
display id: annotation2018439
version: 1
top level: BBa_K235015
location: https://synbiohub.org/public/igem/BBa_K235015/annotation2018439/range2018439/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K235015/annotation2018439/range2018439/1
type: Range
persistent identity: range2018439
display id: range2018439
version: 1
top level: BBa_K235015
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2018444
display id: annotation2018444
version: 1
top level: BBa_K235015
location: https://synbiohub.org/public/igem/BBa_K235015/annotation2018444/range2018444/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K235015/annotation2018444/range2018444/1
type: Range
persistent identity: range2018444
display id: range2018444
version: 1
top level: BBa_K235015
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: iGEM to SBOL conversion
description: AHL-making Enzyme, luxI (+RBS)
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0034.C0061
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: robertb
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
top level: BBa_C0261
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
top level: BBa_C0261
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

https://synbiohub.org/public/igem/BBa_K258012_sequence/1
type: Sequence
persistent identity: BBa K258012 sequence
display id: BBa_K258012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatcagcaaaaacactacggtggataatcagtaaaaccatgaaactagagccccgcacacttgcggggctttttaattttgaatttctttcttattaaaacgccatttttctgataaatgtattagtagcaatgcgcatggtggcatatttgcatcattttgcattttgcaaatgcgatttgcaaaatgcgtgctcaataaagcaccaatatgcatcaggatcgaagaaaaaaggcgtttttaaaagttggcacgcatcgtgctttatacagattactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgtgtaacgacatgacccctgagcaaatggcaacaaacgtgaactgtagtagtcctgaacgccacactcgctcttatgattatatggagggcggggatattcgtgttcgtcgcctgttctgccgtactcaatggtatctgcgcatcgataaacgtggtaaagtgaaaggcacccaagaaatgaaaaacaactataacatcatggaaatccgtaccgtggcagttggtattgtagcgatcaaaggcgtggaatccgagttctatctggcaatgaacaaagagggcaaactgtatgccaaaaaagagtgtaacgaggactgtaacttcaaagaactgatcctggagaaccattataacacctatgccagcgccaaatggacccataacggtggtgaaatgttcgtggccctgaaccaaaaaggcattccggtccgtggcaaaaaaacgaaaaaagagcagaaaaccgcccacttcctgcctatggccattacatactagatgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I751300_sequence/1
type: Sequence
persistent identity: BBa I751300 sequence
display id: BBa_I751300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094130_sequence/1
type: Sequence
persistent identity: BBa K094130 sequence
display id: BBa_K094130_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258006_sequence/1
type: Sequence
persistent identity: BBa K258006 sequence
display id: BBa_K258006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctgggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtggaacgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccgtgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgcgctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggtaatgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccggccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttccagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcagactctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K258004_sequence/1
type: Sequence
persistent identity: BBa K258004 sequence
display id: BBa_K258004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgtaacgacatgacccctgagcaaatggcaacaaacgtgaactgtagtagtcctgaacgccacactcgctcttatgattatatggagggcggggatattcgtgttcgtcgcctgttctgccgtactcaatggtatctgcgcatcgataaacgtggtaaagtgaaaggcacccaagaaatgaaaaacaactataacatcatggaaatccgtaccgtggcagttggtattgtagcgatcaaaggcgtggaatccgagttctatctggcaatgaacaaagagggcaaactgtatgccaaaaaagagtgtaacgaggactgtaacttcaaagaactgatcctggagaaccattataacacctatgccagcgccaaatggacccataacggtggtgaaatgttcgtggccctgaaccaaaaaggcattccggtccgtggcaaaaaaacgaaaaaagagcagaaaaccgcccacttcctgcctatggccattaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K131017_sequence/1
type: Sequence
persistent identity: BBa K131017 sequence
display id: BBa_K131017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtatcagcaaaaacactacggtggataatcagtaaaaccatgaaactagagccccgcacacttgcggggctttttaattttgaatttctttcttattaaaacgccatttttctgataaatgtattagtagcaatgcgcatggtggcatatttgcatcattttgcattttgcaaatgcgatttgcaaaatgcgtgctcaataaagcaccaatatgcatcaggatcgaagaaaaaaggcgtttttaaaagttggcacgcatcgtgctttatacagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K082022_sequence/1
type: Sequence
persistent identity: BBa K082022 sequence
display id: BBa_K082022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235015_sequence/1
type: Sequence
persistent identity: BBa K235015 sequence
display id: BBa_K235015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0261_sequence/1
type: Sequence
persistent identity: BBa C0261 sequence
display id: BBa_C0261_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z