LOCUS       BBa_K258013             1676 bp    DNA     linear       18-Feb-2025
DEFINITION  tetR promoter, EGF and TliA signal tag
ACCESSION   BBa_K258013
VERSION     BBa_K258013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..242
                     /label=BBa_K258010
     misc_feature    249..1676
                     /label=BBa_K258006
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaacagcg actcggagtg tccactgtca catgatggct
      121 attgcctgca cgatggcgtt tgtatgtata tcgaggccct ggacaaatat gcctgtaact
      181 gtgtggtggg ctatatcggt gaacgttgcc agtatcgtga cctgaaatgg tgggaactgc
      241 gttactagat gggtgtgttc gattacaaga acctgggtac ggaagcctct aaaaccctgt
      301 tcgctgatgc gaccgcgatc accctgtata cctaccacaa cctggataac ggtttcgctg
      361 taggttatca gcagcacggc ctgggtctgg gcctgccagc gaccctggtc ggcgcgctgc
      421 tgggttctac cgactctcaa ggcgtgatcc cgggcatccc atggaacccg gactctgaaa
      481 aagcagctct ggacgcggta catgcagctg gctggactcc gatctccgct tctgcgctgg
      541 gctacggtgg caaagtcgac gctcgtggca ccttcttcgg cgaaaaagca ggctatacca
      601 cggcacaggc cgaagtactg ggcaagtacg acgatgctgg taaactgctg gaaatcggca
      661 tcggtttccg tggcaccagc ggtccgcgtg aatccctgat cactgactcc atcggtgatc
      721 tggtaagcga tctgctggcg gctctgggtc cgaaggacta cgcgaaaaac tacgcaggtg
      781 aagccttcgg tggtctgctg aagactgttg cagactacgc cggtgcccac ggtctgtccg
      841 gtaaggacgt cctggtgtcc ggtcatagcc tgggtggtct ggcggtgaac tctatggcag
      901 atctgagcac cagcaagtgg gcgggctttt acaaagatgc aaactacctg gcttacgcct
      961 ctccaacgca gtctgctggt gataaagtcc tgaacatcgg ttacgaaaac gacccggtct
     1021 ttcgtgctct ggacggttcc actttcaacc tgtcctctct gggtgtacac gacaaagccc
     1081 acgaatccac tacggacaac attgtcagct ttaacgacca ctatgcgtct actctgtgga
     1141 acgtcctgcc gttctctatt gccaacctgt ccacttgggt gtctcacctg ccgtctgctt
     1201 acggtgacgg catgacccgt gttctggaaa gcggcttcta cgaacagatg acccgtgatt
     1261 ccactattat tgtggctaac ctgtccgacc cggcgcgcgc taatacgtgg gttcaggatc
     1321 tgaaccgtaa cgcagaaccg cataccggca atactttcat catcggttct gatggtaatg
     1381 acctgattca gggcggtaaa ggtgctgatt tcatcgaggg cggcaaaggt aatgacacta
     1441 tccgtgataa ttccggccac aatacgttcc tgttttccgg tcacttcggc caggaccgta
     1501 ttatcggcta tcagccgacc gaccgtctgg tgttccaggg tgcggacggt agcaccgacc
     1561 tgcgtgacca cgcgaaagcc gtgggcgctg acaccgttct gtctttcggc gcagactctg
     1621 tgactctggt cggcgtcggc ctgggtggcc tgtggtctga aggcgttctg atttct
//