BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K258006 1 BBa_K258006 Thermostable lipase (TliA) of Pseudomonas fluorescens SIK W1 2009-10-08T11:00:00Z 2015-05-08T01:11:42Z The thermostable lipase (TliA) of P. fluorescens SIK W1 is comprised of 476 amino acids and has the characteristic C-terminal signal sequence recognized by the ABC transporter. TliA has four glycinerich repeats (GGXGXD) in its C-terminus, which appear in many ABC transporter-secreted proteins.Export of fusion proteins with the whole TliA through the ABC transporter was evident on the basis of lipase enzymatic activity. Upon supplementation of E. coli with ABC transporter, EGF-TliA was excreted into the culture supernatant.Whole TliA were attached to C-termini of model proteins and enabled the export of the model proteins such as GFP and EGF which has 3 disulfide bonds in E. coli supplemented with ABC transporter. Activity domain (residues 1???268) and secretion/chaperon domain (residues 279???476). In our experiment, we observed that TliA fused proteins were excreted to supernatant culture succesfully by detecting lipase activity with tributyrin and spectrophotometric detection with the substrate p-nitrophenyl phosphate at 420 nm. At the experiments, Tlia fused proteins were excreted ten-fold more with ABC transporter PrtDEF of Erwinia chrysanthemi. false false _358_ 0 4260 9 Not in stock true The sequence was optimized for E.coli with Biobrick restriction sites false Jung Hoon Ahn from Korea Advanced Institute of Science and Technology BBa_K258013 1 BBa_K258013 tetR promoter, EGF and TliA signal tag 2009-10-20T11:00:00Z 2015-05-08T01:11:42Z composite parts Synthesis Epidermal Growth Factor with TliA signal into extraculular environment-supernatant. false false _358_ 0 4260 9 Not in stock false no any consideration false Cihan Tastan component2057811 1 BBa_K258006 component2057810 1 BBa_K258010 component2057809 1 BBa_B0034 component2057803 1 BBa_R0040 annotation2057803 1 BBa_R0040 range2057803 1 1 54 annotation2057811 1 BBa_K258006 range2057811 1 249 1676 annotation2057810 1 BBa_K258010 range2057810 1 81 242 annotation2057809 1 BBa_B0034 range2057809 1 63 74 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986785 1 -35 range1986785 1 20 25 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986783 1 TetR 1 range1986783 1 1 19 BBa_K258010 1 BBa_K258010 Human Epidermal growth factor (hEGF) 2009-10-09T11:00:00Z 2015-05-08T01:11:42Z EGF is synthesized in human and some mammalians. Our EGF was synthesized synthetically at GENEART Epidermal growth factor (EGF) is a single chain, 53 aminoacid-residue polypeptide (molecular weight 6045) and three intramolecular disulfide bonds. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. EGF results in cellular proliferation, differentiation, and survival. EGF stimulates the growth of skin and corneal epithelium in vivo and in organ cultures. Epidermal growth factor (EGF) stimulates the growth of keratinocytes in vivo, and therefore plays an important role in the process of wound healing that depends on mitosis and migration of keratinocytes. Rhinewald and Green showed, in vitro that in the presence of growth factors , higher percentage of cells leave the resting state, enter and remain in the mitotic cycle. Mitogenic effect of EGF requires continuous exposure of target cells to EGF for a minimum 6-12 hours. The stimulation of wound healing by EGF has been confirmed as growth of granulation tissue in sponge implants used as inductive matrices. They observed, in rats, dose-dependent effect of EGF on granulation tissue formation as increased amounts of nucleic acids, and accumulation of collagen and glycosaminoglycans. false false _358_ 0 4260 9 It's complicated true EGF with Biobrick standart restriction sites false Cihan Tastan and Ozkan IS BBa_K258013_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacagcgactcggagtgtccactgtcacatgatggctattgcctgcacgatggcgtttgtatgtatatcgaggccctggacaaatatgcctgtaactgtgtggtgggctatatcggtgaacgttgccagtatcgtgacctgaaatggtgggaactgcgttactagatgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctgggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtggaacgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccgtgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgcgctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggtaatgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccggccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttccagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcagactctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttct BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K258006_sequence 1 atgggtgtgttcgattacaagaacctgggtacggaagcctctaaaaccctgttcgctgatgcgaccgcgatcaccctgtatacctaccacaacctggataacggtttcgctgtaggttatcagcagcacggcctgggtctgggcctgccagcgaccctggtcggcgcgctgctgggttctaccgactctcaaggcgtgatcccgggcatcccatggaacccggactctgaaaaagcagctctggacgcggtacatgcagctggctggactccgatctccgcttctgcgctgggctacggtggcaaagtcgacgctcgtggcaccttcttcggcgaaaaagcaggctataccacggcacaggccgaagtactgggcaagtacgacgatgctggtaaactgctggaaatcggcatcggtttccgtggcaccagcggtccgcgtgaatccctgatcactgactccatcggtgatctggtaagcgatctgctggcggctctgggtccgaaggactacgcgaaaaactacgcaggtgaagccttcggtggtctgctgaagactgttgcagactacgccggtgcccacggtctgtccggtaaggacgtcctggtgtccggtcatagcctgggtggtctggcggtgaactctatggcagatctgagcaccagcaagtgggcgggcttttacaaagatgcaaactacctggcttacgcctctccaacgcagtctgctggtgataaagtcctgaacatcggttacgaaaacgacccggtctttcgtgctctggacggttccactttcaacctgtcctctctgggtgtacacgacaaagcccacgaatccactacggacaacattgtcagctttaacgaccactatgcgtctactctgtggaacgtcctgccgttctctattgccaacctgtccacttgggtgtctcacctgccgtctgcttacggtgacggcatgacccgtgttctggaaagcggcttctacgaacagatgacccgtgattccactattattgtggctaacctgtccgacccggcgcgcgctaatacgtgggttcaggatctgaaccgtaacgcagaaccgcataccggcaatactttcatcatcggttctgatggtaatgacctgattcagggcggtaaaggtgctgatttcatcgagggcggcaaaggtaatgacactatccgtgataattccggccacaatacgttcctgttttccggtcacttcggccaggaccgtattatcggctatcagccgaccgaccgtctggtgttccagggtgcggacggtagcaccgacctgcgtgaccacgcgaaagccgtgggcgctgacaccgttctgtctttcggcgcagactctgtgactctggtcggcgtcggcctgggtggcctgtggtctgaaggcgttctgatttct BBa_K258010_sequence 1 atgaacagcgactcggagtgtccactgtcacatgatggctattgcctgcacgatggcgtttgtatgtatatcgaggccctggacaaatatgcctgtaactgtgtggtgggctatatcggtgaacgttgccagtatcgtgacctgaaatggtgggaactgcgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z