BBa_K258015 1 BBa_K258015 Vhb protein 2009-10-20T11:00:00Z 2015-05-08T01:11:42Z gene coding for the Vitreoscilla hemoglobin (VHb) The gene coding for the Vitreoscilla hemoglobin (VHb) molecule has been cloned and functionally expressed in Escherichia coli. By using a plasmid-encoded gene as well as single-copy integrants, the oxygen-dependent VHb gene (VHb) promoter was shown to be functional in E. coli. The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation). We wanted to measure the activity of VHb gene; therefore, the VHb promoter. The VHb promoter becomes active when the surrounding oxygen amount lowers to 2%. We wanted to see whether this promoter will effectively work with our E. Coli cells. false true _358_ 0 4260 9 Not in stock false no any consideration false Cihan Tastan component2059282 1 BBa_K258005 component2059284 1 BBa_B0034 annotation2059282 1 BBa_K258005 range2059282 1 1 137 annotation2059284 1 BBa_B0034 range2059284 1 146 157 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K258005 1 BBa_K258005 Oxygen promoter-Vitreoscilla hemoglobin(VHb) promoter in E. coli. 2009-10-03T11:00:00Z 2015-05-08T01:11:42Z gene of Vitreoscilla hemoglobin(VHb). We synthesized synthetically at GENEART. The promoter was maximally induced under microaerobic conditions (dissolved oxygen levels of less than 2% air saturation). Transcriptional activity decreased substantially under anaerobic conditions, suggesting the presence of a regulatory mechanism that is maximally induced under hypoxic but not completely anaerobic conditions in E.coli. Primer extension analysis was used to identify the existence of two overlapping promoterswithin a 150-base-pair region upstream of the structural VHb gene. The oxygen-dependent activity of both promoters was qualitatively similar, suggesting the existence of a common mechanism by which available oxygen concentrations influence expression from the two promoters. Oxygen-dependent control mechanisms revealed in some of the above studies include positive regulation by an activator protein. The bacterium Vitreo-scilla sp. is an obligate aerobe from the Beggiatoa family that synthesizes a hemoglobinlike molecule (VHb) in response to growth in oxygen-poor environments. The expression of the VHb gene (vgb) is regulated by oxygen in both its native host, Vitreoscilla, and in E.coli and is maximally induced under microaerophilic conditions (Dikshit and Webster 1988; Dikshit et al. 1992;Joshi and Dikshit 1994). The purposes were to characterize the response of the promoter to changes in oxygen availability in the environment and to obtain initial insights about the mechanism(s) by the promoter is controlled. false false _358_ 0 4260 9 It's complicated true The palsmid id pMA (GENEART constantpalsmid with Amphicillin resistance and Biobrick restiction sites,EcoR1,Xba1,Spe1 and Pst1) false Cihan Tastan BBa_B0034_sequence 1 aaagaggagaaa BBa_K258005_sequence 1 aagcttacaggacgctggggttaaagtatttgagttttgatgtggattaagtttagaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaccataataatgaacttaaggaagaccctc BBa_K258015_sequence 1 aagcttacaggacgctggggttaaagtatttgagttttgatgtggattaagtttagaggcaataaagattataataagtgctgctacaccatactgatgtatggcaaaccataataatgaacttaaggaagaccctctactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z