BBa_K259002
1
BioScaffol
BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker
2009-07-31T11:00:00Z
2015-05-08T01:11:42Z
This part was designed ''de novo''. The sequence does not occur naturally.
This part is part of a family of Bioscaffold - Linkers.
This part will help users interested in protein-fusion. The usage of the part will allow in frame assembly of 2(+) proteins by:
*Removing the stop codons from the upstream protein.
*Removing the scar created by the Assembly Standard 10 (RFC10) construction of 2(+) protein coding sequences.
*Maintaining the start codon of the downstream protein.
*Adding a flexible (Gly-Ser-Gly) linker between the two protein coding sequences.
*Acting as a translational buffer if ligation is incorrect.
===Assembly Instructions====
*Create an Assembly Standard 10 fusion giving ;
**Upstream Protein Part - Bioscaffold Linker - Downstream Protein Part
*Use the BioScaffold Specific Enzymes
**Restrict with BpuEI to remove upstream scar (created between Upstream Protein - Bioscaffold) and stop codons from upstream protein part.
**Ligate and you will get the intermediate product;
Upstream Protein (w/out Stop codons) - Tyr - BioScaffold Linker - Downstream Protein
**Restrict with BseRI to remove downstream scar.
**Ligate to get the final product;
Upstream Protein - Tyr-Gly-Ser-Gly- Downstream Protein
false
false
_357_
0
5318
9
It's complicated
false
Part was designed taking into account RFC10 assembly standard and as an attempt to be upwards compatible with RFC10.
false
Petros Mina
annotation2014595
1
BseRI Site
range2014595
1
63
68
annotation2014592
1
Gly-Ser-Gly Linker
range2014592
1
44
52
annotation2014596
1
BseRI Site
range2014596
1
81
86
annotation2014593
1
Met involved in Restriction/Ligation
range2014593
1
53
55
annotation2014590
1
BpuEI Site
range2014590
1
3
8
annotation2014594
1
Translational Buffer
range2014594
1
56
62
annotation2014591
1
BpuEI Site
range2014591
1
21
26
annotation2014589
1
Tyr Residue involved in Restriction/Ligation
range2014589
1
41
43
BBa_K259002_sequence
1
atctcaagagtggcagcggtcttgagtggcagcggcggtatacggcagcggtatgtaactagctcctcagtggcagcggtgaggaggc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z