##gff-version 3 ##sequence-region BBa_K259009 3 77 BBa_K259009 . stop_codon 56 65 . + 0 ID=annotation2037405;Name=Translational Buffer BBa_K259009 . sequence_feature 3 8 . + 0 ID=annotation2037400;Name=BpuEI (rev. complement) BBa_K259009 . sequence_feature 74 77 . + 0 ID=annotation2037438;Name=3' CspCI recognition sequence BBa_K259009 . sequence_feature 44 52 . + 0 ID=annotation2037403;Name=Gly-Ser-Gly linker BBa_K259009 . sequence_feature 41 43 . + 0 ID=annotation2037402;Name=Tyr involved in restriction/ligation BBa_K259009 . stop_codon 69 73 . + 0 ID=annotation2037439;Name=Translational Buffer BBa_K259009 . sequence_feature 66 68 . + 0 ID=annotation2037437;Name=5' CspCI Recognition sequence BBa_K259009 . sequence_feature 53 55 . + 0 ID=annotation2037404;Name=Met involved in restriction/ligation BBa_K259009 . sequence_feature 21 26 . + 0 ID=annotation2037401;Name=BpuEI >BBa_K259009 atctcaagagtggcagcggtcttgagtggcagcggcggtatacggcagcggtatgtaagtagtaacaagtagcgtgggg cag