LOCUS       BBa_K259013             1189 bp    DNA     linear       22-Dec-2024
DEFINITION  AraC-RBS-GFP-Terminator
ACCESSION   BBa_K259013
VERSION     BBa_K259013.1  
FEATURES             Location/Qualifiers
     CDS             1..78
                     /label=defunct araC
     stem_loop       22..37
                     /label=stem_loop
     stem_loop       57..72
                     /label=stem_loop
     promoter        1..183
                     /label=BBa_R0081
     misc_feature    195..220
                     /label=c-amp2
     misc_feature    197..235
                     /label=araO1
     misc_feature    234..263
                     /label=c-amp1
     misc_feature    264..292
                     /label=ara1 and ara2
     promoter        304..309
                     /label=-35
     promoter        327..332
                     /label=-10
     promoter        192..340
                     /label=BBa_R0080
     RBS             349..360
                     /label=BBa_J61100
     CDS             367..1086
                     /label=GFP protein
     CDS             367..1086
                     /label=BBa_E0040
     stem_loop       1102..1121
                     /label=T7 TE
     misc_feature    1128..1128
                     /label=stop
     polyA_site      1122..1135
                     /label=polya
     misc_feature    1095..1135
                     /label=BBa_B0012
     terminator      1095..1135
                     /label=BBa_B0012
     stem_loop       1156..1178
                     /label=stem_loop
     misc_feature    1144..1189
                     /label=BBa_B0011
     terminator      1144..1189
                     /label=BBa_B0011
ORIGIN
        1 caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca gagaagaaac
       61 caattgtcca tattgctaca gacattgccg tcactggtct ttactggctc ttctcgctaa
      121 ccaaaccggt aaccgcttat taaagcattc tgtaacaaag cgggaccaaa gccatgacaa
      181 aactactaga ggcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
      241 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
      301 acctgacgct ttttatcgca actctctact gtttctccat tactagagaa agaggggaca
      361 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      421 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      481 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      541 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      601 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      661 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      721 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      781 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      841 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      901 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      961 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1021 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1081 taataatact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1141 gagagagaat ataaaaagcc agattattaa tccggctttt ttattattt
//