LOCUS       BBa_K260009             2599 bp    DNA     linear       23-Dec-2024
DEFINITION  pMA-RQ-@TetFlp
ACCESSION   BBa_K260009
VERSION     BBa_K260009.1  
FEATURES             Location/Qualifiers
     stem_loop       22..60
                     /label=BBa_B1006 (terminator)
     misc_feature    1..21
                     /label=suffix
     misc_feature    67..136
                     /label=BBa_K260002 5' homology
     misc_feature    2502..2571
                     /label=BBa_K260002 3' homology
     misc_feature    317..984
                     /label=ColE1 origin
     CDS             1132..1992
                     /label=AmpR
     misc_feature    137..142
                     /label=PvuII site
     misc_feature    2496..2501
                     /label=PvuII site
     misc_feature    2578..2599
                     /label=prefix
ORIGIN
        1 tactagtagc ggccgctgca gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt
       61 ctcgagtcta gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc
      121 attagccagt ctgaatcagc tggagctcct gggcctcatg ggccttccgc tcactgcccg
      181 ctttccagtc gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg
      241 cgtattgggc gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa
      301 agcctggggt gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      781 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1261 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1381 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1441 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1501 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1561 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1621 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1681 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1741 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1801 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1861 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1921 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1981 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2041 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2101 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     2161 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2221 aaatcaaaag aatagaccga gatagggttg agtggccgct acagggcgct cccattcgcc
     2281 attcaggctg cgcaactgtt gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc
     2341 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2401 agtcacgacg ttgtaaaacg acggccagtg agcgcgacgt aatacgactc actatagggc
     2461 gaattggcgg aaggccgtca aggccgcatg gtacccagct gatgatagag tgtcaacaaa
     2521 aattaggaat taatcatctg gccattcgat ggtgtcgggt gatatcaatt cctcgaggaa
     2581 ttcgcggccg cttctagag
//