BBa_K260009 1 BBa_K260009 pMA-RQ-@TetFlp 2009-10-17T11:00:00Z 2015-05-08T01:11:42Z Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp [[Part:BBa_K260002|]]. This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology to pTetFlp ([[Part:BBa_K260002|]]) for tranfer of the contained BioBrick part by recombineering, for which the BioBrick needs to be excised with PvuII from the backbone. This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP ([[Part:BBa_K260017|]]). The product of the above-described recombineering reaction [[Part:BBa_K260002|]]-[[Part:BBa_K260017|]] is also available. false false _356_ 0 1072 57 Not in stock true The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone '''pMA-RQ-@TetFlp''' to pTetFlp [[Part:BBa_K260002|]] via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched. false Kaj Bernhardt annotation2049534 1 suffix range2049534 1 1 21 annotation2049681 1 prefix range2049681 1 2578 2599 annotation2049652 1 PvuII site range2049652 1 2496 2501 annotation2049595 1 PvuII site range2049595 1 137 142 annotation2049593 1 ColE1 origin range2049593 1 317 984 annotation2049594 1 AmpR range2049594 1 1132 1992 annotation2049591 1 BBa_K260002 5' homology range2049591 1 67 136 annotation2049592 1 BBa_K260002 3' homology range2049592 1 2502 2571 annotation2049496 1 BBa_B1006 (terminator) range2049496 1 22 60 BBa_K260009_sequence 1 tactagtagcggccgctgcagaaaaaaaaaccccgcccctgacagggcggggttttttttctcgagtctagacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatcagctggagctcctgggcctcatgggccttccgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaacatggtcatagctgtttccttgcgtattgggcgctctccgcttcctcgctcactgactcgctgcgctcggtcgttcgggtaaagcctggggtgcctaatgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaaccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtggccgctacagggcgctcccattcgccattcaggctgcgcaactgttgggaagggcgtttcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgacgtaatacgactcactatagggcgaattggcggaaggccgtcaaggccgcatggtacccagctgatgatagagtgtcaacaaaaattaggaattaatcatctggccattcgatggtgtcgggtgatatcaattcctcgaggaattcgcggccgcttctagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z