LOCUS       BBa_K260010             3097 bp    DNA     linear       23-Dec-2024
DEFINITION  pMA-BsdR-@CC2FosM
ACCESSION   BBa_K260010
VERSION     BBa_K260010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=suffix
     misc_feature    2149..2816
                     /label=ColE1 origin
     promoter        22..115
                     /label=gb3 promoter
     CDS             116..514
                     /label=optimised BsdR
     stem_loop       515..553
                     /label=BBa_B1006 (terminator)
     misc_feature    554..623
                     /label=BBa_K260000 3' homology
     misc_feature    624..629
                     /label=HincII site
     CDS             1141..2001
                     /label=AmpR
     misc_feature    3000..3005
                     /label=HincII site
     misc_feature    3006..3075
                     /label=BBa_K260000 5' homology
     misc_feature    3076..3097
                     /label=prefix
ORIGIN
        1 tactagtagc ggccgctgca gtatggccac aacctgttac attgcacaag ataaaaatat
       61 atcatcacga acagtaaaac tgtctgctta cataaacagt aatacaaggg gtgttatggc
      121 caagccactg tctcaagaag aatccaccct gattgaaaga gcaactgcaa ccatcaacag
      181 catcccaatc tctgaagact attctgttgc ctctgctgca ctgtcttctg atggcagaat
      241 cttcactggt gtcaatgtat atcattttac tggtggccct tgtgcagaac tggttgtgct
      301 gggcacagca gcagcagctg ctgctggcaa cctgacttgt attgttgcca ttggtaatga
      361 gaacagaggc atcctgagcc catgtggcag atgcagacag gtgctgctgg atctgcatcc
      421 tggcatcaaa gctattgtga aggactctga tggtcagcca actgctgttg gcattagaga
      481 actgctgcca tctggttatg tgtgggaggg ctgaaaaaaa aaaccccgcc cctgacaggg
      541 cggggttttt tttagtaggc aaggctggct caacattcaa aaatcaatta aagaggccgg
      601 gcgcggtggc tcacgcctgt catgttaacg agctcatggc gcgcctaggc cttgacggcc
      661 ttccgccaat tcgccctata gtgagtcgta ttacgtcgcg ctcactggcc gtcgttttac
      721 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      781 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga aacgcccttc ccaacagttg
      841 cgcagcctga atggcgaatg ggagcgccct gtagcggcca ctcaacccta tctcggtcta
      901 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
      961 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     1021 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1141 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1201 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1261 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1321 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1381 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1441 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1501 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1561 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1621 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1681 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1741 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1801 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct
     1861 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1921 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1981 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2041 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2101 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2281 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2521 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2761 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcattag
     2821 gcaccccagg ctttacccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaga
     2881 gcgcccaata cgcaaggaaa cagctatgac catgttaatg cagctggcac gacaggtttc
     2941 ccgactggaa agcgggcagt gagcggaagg cccatgaggc cagttaatta agaggtaccg
     3001 ttaactctat gaggctagca tcaccttgat gtcaaaacca gaagacatac caagaaaact
     3061 atagaccaat atcccgaatt cgcggccgct tctagag
//