##gff-version 3
##sequence-region BBa_K260010 1 3097
BBa_K260010	.	engineered_region	1	21	.	+	0	ID=annotation2049721;Name=suffix
BBa_K260010	.	stem_loop	515	553	.	+	0	ID=annotation2049725;Name=BBa_B1006 (terminator)
BBa_K260010	.	sequence_feature	3006	3075	.	+	0	ID=annotation2049730;Name=BBa_K260000 5' homology
BBa_K260010	.	sequence_feature	2149	2816	.	+	0	ID=annotation2049722;Name=ColE1 origin
BBa_K260010	.	CDS	1141	2001	.	+	0	ID=annotation2049728;Name=AmpR
BBa_K260010	.	sequence_feature	554	623	.	+	0	ID=annotation2049726;Name=BBa_K260000 3' homology
BBa_K260010	.	promoter	22	115	.	+	0	ID=annotation2049723;Name=gb3 promoter
BBa_K260010	.	sequence_feature	624	629	.	+	0	ID=annotation2049727;Name=HincII site
BBa_K260010	.	sequence_feature	3000	3005	.	+	0	ID=annotation2049729;Name=HincII site
BBa_K260010	.	CDS	116	514	.	+	0	ID=annotation2049724;Name=optimised BsdR
BBa_K260010	.	engineered_region	3076	3097	.	+	0	ID=annotation2049731;Name=prefix
>BBa_K260010
tactagtagcggccgctgcagtatggccacaacctgttacattgcacaagataaaaatatatcatcacgaacagtaaaa
ctgtctgcttacataaacagtaatacaaggggtgttatggccaagccactgtctcaagaagaatccaccctgattgaaa
gagcaactgcaaccatcaacagcatcccaatctctgaagactattctgttgcctctgctgcactgtcttctgatggcag
aatcttcactggtgtcaatgtatatcattttactggtggcccttgtgcagaactggttgtgctgggcacagcagcagca
gctgctgctggcaacctgacttgtattgttgccattggtaatgagaacagaggcatcctgagcccatgtggcagatgca
gacaggtgctgctggatctgcatcctggcatcaaagctattgtgaaggactctgatggtcagccaactgctgttggcat
tagagaactgctgccatctggttatgtgtgggagggctgaaaaaaaaaaccccgcccctgacagggcggggtttttttt
agtaggcaaggctggctcaacattcaaaaatcaattaaagaggccgggcgcggtggctcacgcctgtcatgttaacgag
ctcatggcgcgcctaggccttgacggccttccgccaattcgccctatagtgagtcgtattacgtcgcgctcactggccg
tcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccag
ctggcgtaatagcgaagaggcccgcaccgaaacgcccttcccaacagttgcgcagcctgaatggcgaatgggagcgccc
tgtagcggccactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaa
aaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggg
gaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctg
ataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg
gcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgag
tgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgag
cacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacac
tattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattat
gcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaac
cgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaac
gacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctag
cttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctgg
ctggtttattgctgataaatctggagccggtgagcgtggttctcgcggtatcattgcagcactggggccagatggtaag
ccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagatag
gtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcattt
ttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccac
tgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaa
caaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctt
cagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccg
cctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggact
caagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaac
gacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacagg
tatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtc
ctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgc
cagcaacgcggcctttttacggttcctggccttttgctggccttttgctcattaggcaccccaggctttacccgaacga
ccgagcgcagcgagtcagtgagcgaggaagcggagagcgcccaatacgcaaggaaacagctatgaccatgttaatgcag
ctggcacgacaggtttcccgactggaaagcgggcagtgagcggaaggcccatgaggccagttaattaagaggtaccgtt
aactctatgaggctagcatcaccttgatgtcaaaaccagaagacataccaagaaaactatagaccaatatcccgaattc
gcggccgcttctagag