LOCUS       BBa_K260016              882 bp    DNA     linear       01-Jul-2024
DEFINITION  TT_FRT_GFP. (double terminator, FRT site, optimised GFP)
ACCESSION   BBa_K260016
VERSION     BBa_K260016.1  
FEATURES             Location/Qualifiers
     stem_loop       1..129
                     /label=BBa_B0015 (double terminator)
     misc_feature    131..164
                     /label=translated FRT site
     CDS             166..882
                     /label=optimised GFP (mutb3)
     misc_feature    1..882
                     /label=BBa_K260016
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat gaagttccta ttctctagaa agtataggaa cttctgcaag caaaggtgaa
      181 gaactgttca ctggtgttgt cccaattctg gttgagctgg atggtgatgt gaatggccac
      241 aaattttctg tgtctggtga aggtgagggt gatgcaactt atggcaaact gactctgaaa
      301 tttatctgta ccactggcaa actgcctgtt ccatggccaa ctctggtcac cactttctct
      361 tatggtgtgc agtgttttgc aagataccca gatcacatga aacagcatga cttcttcaaa
      421 tctgccatgc ctgaaggcta tgtccaagaa agaaccatct ctttcaagga tgatggtaac
      481 tataaaacca gagctgaagt taaatttgag ggtgacaccc tggtgaacag aattgaactg
      541 aaaggcattg acttcaaaga ggatggcaac attctgggtc acaagctgga atacaactat
      601 aactcccaca atgtttacat tactgctgac aagcagaaga atggcatcaa agcaaacttc
      661 aagatcagac acaacattga agatggtggt gtacaactgg cagatcacta tcagcagaac
      721 actcctattg gtgatggccc agtactgctg ccagataacc attacctgtc cacccagagc
      781 aaactgtcta aagacccaaa tgaaaagaga gaccacatgg tactgctgga atttgttact
      841 gctgctggca ttacccatgg tatggatgaa ctgtataaat ga
//