LOCUS       BBa_K260017             1309 bp    DNA     linear       22-Nov-2024
DEFINITION  P_F3_ZeoR_F3_RFP.   [FLP]->RFP
ACCESSION   BBa_K260017
VERSION     BBa_K260017.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100 (strong promoter)
     RBS             44..55
                     /label=BBa_B0034 (Elowitz RBS)
     misc_feature    62..64
                     /label=ATG-Start
     misc_feature    66..99
                     /label=translated F3 site
     CDS             101..469
                     /label=optimised ZeoR
     stem_loop       470..598
                     /label=BBa_B0015 (double terminator)
     misc_feature    600..633
                     /label=F3 site
     CDS             635..1309
                     /label=optimised mRFP1 (without Start-codon)
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgggaagt tcctattctt caaatagtat aggaacttct aagctgacct ctgctgttcc
      121 agtgctgact gctagagatg tggcaggtgc tgttgagttc tggactgaca gactgggctt
      181 ctccagagac tttgtggagg atgactttgc aggtgtggtt agagatgatg tgaccctgtt
      241 catctctgct gtccaggacc aggtggtgcc agacaacacc ctggcctggg tgtgggtgag
      301 aggcctggat gagctgtatg ctgagtggtc tgaggttgtg tccactaact tcagagatgc
      361 ctctggccca gccatgactg agattggtga gcagccttgg ggcagagagt ttgccctgag
      421 agacccagca ggcaactgtg tgcactttgt ggctgaggag caggactgac caggcatcaa
      481 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      541 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatatg
      601 aagttcctat tcttcaaata gtataggaac ttctgcctcc tctgaggatg tcatcaagga
      661 gttcatgaga ttcaaagtta gaatggaggg ttctgttaat ggtcatgagt ttgagattga
      721 aggtgaaggt gagggcagac catatgaagg cacccagact gccaaactga aagtgactaa
      781 gggtggccct ctgccatttg cttgggacat cctgagccct caatttcagt atggttctaa
      841 agcatatgtt aaacaccctg ctgatatccc tgactatctg aagctgtctt ttcctgaagg
      901 cttcaagtgg gaaagagtca tgaattttga agatggtggt gtggtcactg tcacccagga
      961 cagctccctg caagatggtg agttcatcta taaagttaaa ctgagaggta ctaattttcc
     1021 atctgatggc cctgtgatgc agaagaagac catgggttgg gaggccagca ctgaaagaat
     1081 gtatcctgaa gatggtgccc tgaaaggtga aatcaaaatg agactgaaac tgaaagatgg
     1141 tggccattat gatgctgaag tgaaaaccac ttatatggcc aagaaacctg tgcaactgcc
     1201 tggtgcttac aaaactgata tcaaactgga catcacctct cataatgaag attataccat
     1261 tgtggagcaa tatgagagag ctgagggcag acatagcact ggtgcctga
//