LOCUS       BBa_K264018              936 bp    DNA     linear       25-Nov-2024
DEFINITION  Up Element BBa_K264001/Constitutive promoter BBa_J23119/RFP 
            reporter BBa_J06702
ACCESSION   BBa_K264018
VERSION     BBa_K264018.1  
FEATURES             Location/Qualifiers
     promoter        1..59
                     /label=BBa_K264041
     misc_feature    72..75
                     /label=conserved
     RBS             68..79
                     /label=BBa_B0034
     CDS             86..796
                     /label=mCherry
     misc_feature    437..437
                     /label=C->T (removing PstI site)
     misc_feature    86..799
                     /label=BBa_J06504
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
ORIGIN
        1 taaaattttt ttttgaaaag tactttgaca gctagctcag tcctaggtat aatgctagct
       61 actagagaaa gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat
      121 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      181 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      241 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      301 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
      361 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
      421 gacccaggac tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      481 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
      541 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
      601 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
      661 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
      721 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      781 cgagctgtac aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttata
//