BBa_K264003
1
BBa_K264003
Modular UP-element Design 4
2009-10-18T11:00:00Z
2015-05-08T01:11:42Z
Rational redesign of BBa_K264000
The UP element is a component of the promoter located upstream of the -35 region. Though not necessary for transcription, the UP element increases the rate of transcription initiation by interacting with the alpha-subunit of RNA polymerase. A consensus sequence, -59 nnAAA(A/T)(A/T)T(A/T)TTTTnnAAAAnnn -38, was derived from a collection of many UP elements based on nucleotide frequency at each position (Estrem et al., 1998). UP Element Design 1 is the UP element with the highest transcription rate increase reported in that study. Design 1 was systematically modified (BBa_K264001-K264007) to vary the relative strength of its effect based on the hypothesis that the most significant promoter enhancement would occur when the UP element sequence used the most frequently-occurring nucleotides at each position (i.e., the consensus sequence). A spacer, CT, is placed between the UP element and subsequent promoters to maintain proper alignment with RNAP.
false
false
_364_
0
5271
9
Not in stock
false
The observed UP elements were A-T rich. Therefore, the rational sequence modifications of Design 1 were made to preserve this feature. Nucleotides in Design 1 at positions -59, -58, -54, and -45 were changed from G to A, G to A, A to T, and C to G, respectively. A spacer, CT, is placed between the UP element and subsequent promoters to maintain proper alignment with RNAP.
false
Adam Bower
BBa_J23112
1
BBa_J23112
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Released HQ 2013
Later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K264050
1
BBa_K264050
K264003.J23112 (UP/promoter)
2009-11-10T12:00:00Z
2015-05-08T01:11:43Z
This is a composite part.
This engineered part is a combination of UP element BBa_K264003 and constitutive promoter BBa_J23112.
false
false
_364_
0
2920
9
Not in stock
false
A spacer, CT, was placed at the end of the UP-element to serve as a 2 bp spacer between the UP-element and promoter. There is no scar between the UP-element and promoter.
false
George McArthur IV
component2062626
1
BBa_K264003
component2062627
1
BBa_J23112
annotation2062626
1
BBa_K264003
range2062626
1
1
24
annotation2062627
1
BBa_J23112
range2062627
1
25
59
BBa_J23112_sequence
1
ctgatagctagctcagtcctagggattatgctagc
BBa_K264050_sequence
1
aaaaatttttttttgaaaagtactctgatagctagctcagtcctagggattatgctagc
BBa_K264003_sequence
1
aaaaatttttttttgaaaagtact
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z