BBa_K265011
1
BBa_K265011
OmpA with proteolytic cleavage tag
2009-10-14T11:00:00Z
2015-05-08T01:11:43Z
The OmpA was part BBa_K103006 with a couple modified restriction sites. The signal sequence was synthesized by Mr. Gene.
This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with Tom Knight's fusion format.
An OmpA/protein fusion normally allows display of proteins on the cell surface. The addition of this signal sequence allows secretion by allowing cleavage after display.
false
false
_362_
0
4713
9
It's complicated
true
None
false
Richard Hauser
annotation2047286
1
Proteolytic Cleavage Sequence
range2047286
1
471
584
annotation2047259
1
NdeI
range2047259
1
1
7
annotation2047264
1
BBa_K265002
range2047264
1
471
584
annotation2047262
1
OmpA
range2047262
1
4
438
annotation2047263
1
linker
range2047263
1
439
464
annotation2047261
1
ATG
range2047261
1
4
6
annotation2047260
1
BBa_K103006
range2047260
1
1
464
BBa_K265011_sequence
1
catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctcgctagtatgctggccaagcagatcaagaaggctaatagccgctctacactgctacgcaaatcactgttattcgcagcccctatcatcttagcagtttcatcgtcctccgtatatgcgctg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z