##gff-version 3
##sequence-region BBa_K266001 1 1890
BBa_K266001	.	engineered_region	1	883	.	+	0	ID=BBa_K145201;Name=BBa_K145201
BBa_K266001	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K266001	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K266001	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K266001	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K266001	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K266001	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K266001	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K266001	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K266001	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266001	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266001	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266001	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266001	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266001	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266001	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266001	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266001	.	engineered_region	892	1890	.	+	0	ID=BBa_K116640;Name=BBa_K116640
BBa_K266001	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K266001	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K266001	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K266001	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K266001	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K266001	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K266001	.	engineered_region	954	1890	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K266001	.	engineered_region	954	1753	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K266001	.	CDS	973	1728	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K266001	.	engineered_region	973	1728	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K266001	.	CDS	973	1722	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K266001	.	point_mutation	1146	1146	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K266001	.	point_mutation	1464	1464	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K266001	.	engineered_tag	1729	1753	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K266001	.	engineered_region	973	974	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K266001	.	ribosome_entry_site	954	966	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K266001	.	ribosome_entry_site	960	963	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K266001	.	ribosome_entry_site	954	966	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K266001	.	engineered_region	954	966	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K266001	.	terminator	1762	1890	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K266001	.	terminator	1850	1890	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266001	.	engineered_region	1850	1890	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266001	.	stop_codon	1883	1883	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266001	.	stem_loop	1857	1876	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266001	.	polyA_site	1877	1890	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266001	.	terminator	1762	1841	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266001	.	stem_loop	1773	1816	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266001	.	engineered_region	1762	1841	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K266001
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagctt
gtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgat
catttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgat
gacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttg
aaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagttt
ccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagttta
tttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaata
ataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatat
ttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaac
cgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagt
gctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata