##gff-version 3
##sequence-region BBa_K266004 1 2358
BBa_K266004	.	engineered_region	44	2358	.	+	0	ID=BBa_K266003;Name=BBa_K266003
BBa_K266004	.	ribosome_entry_site	1423	1434	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K266003
BBa_K266004	.	conserved_region	1427	1430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K266004	.	CDS	1441	2358	.	+	0	ID=BBa_K266002;Name=BBa_K266002;Parent=BBa_K266003
BBa_K266004	.	CDS	1441	2221	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_K266002
BBa_K266004	.	engineered_tag	2197	2221	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K266004	.	CDS	1441	2157	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K266004	.	sequence_alteration	1920	1920	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K266004	.	tag	2158	2190	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K266004	.	terminator	2230	2358	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K266002
BBa_K266004	.	terminator	2318	2358	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266004	.	stem_loop	2325	2344	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266004	.	engineered_region	2318	2358	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266004	.	stop_codon	2351	2351	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266004	.	polyA_site	2345	2358	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266004	.	terminator	2230	2309	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266004	.	engineered_region	2230	2309	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266004	.	stem_loop	2241	2284	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266004	.	engineered_region	44	1414	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_K266003
BBa_K266004	.	terminator	1223	1351	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K266004	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266004	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266004	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266004	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266004	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266004	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266004	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266004	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266004	.	promoter	1360	1413	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K266004	.	operator	1385	1401	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K266004	.	engineered_region	1360	1413	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K266004	.	operator	1362	1378	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K266004	.	promoter	1379	1384	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K266004	.	promoter	1402	1407	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K266004	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K266004	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K266004	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K266004	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K266004	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K266004	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K266004	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K266004	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K266004
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagag
aaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcg
ccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggacta
cgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgac
ccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacg
agttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcgg
cgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtgg
atgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgacca
gccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcgga
agccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggcc
gttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata