LOCUS       BBa_K266006             1705 bp    DNA     linear       22-Dec-2024
DEFINITION  PAI+LasR -> LasI+GFP  &  AI+LuxR --| LasI+GFP
ACCESSION   BBa_K266006
VERSION     BBa_K266006.1  
FEATURES             Location/Qualifiers
     misc_feature    75..93
                     /label=LasR
     misc_feature    94..113
                     /label=LuxR
     misc_feature    113..118
                     /label=-10
     misc_feature    86..91
                     /label=-35
     misc_feature    125..125
                     /label=Transcriptional Start
     promoter        1..126
                     /label=BBa_K091146
     misc_feature    139..142
                     /label=conserved
     RBS             135..146
                     /label=BBa_B0034
     CDS             153..755
                     /label=LasI
     misc_feature    756..788
                     /label=LVA
     misc_feature    795..819
                     /label=Help:Barcodes
     CDS             153..794
                     /label=BBa_C0078
     misc_feature    135..819
                     /label=BBa_S03154
     misc_feature    1..819
                     /label=BBa_K266005
     RBS             828..842
                     /label=RBS-1\Strong
     RBS             835..839
                     /label=RBS
     misc_feature    828..842
                     /label=BBa_B0030
     RBS             828..842
                     /label=BBa_B0030
     CDS             849..1568
                     /label=GFP protein
     CDS             849..1568
                     /label=BBa_E0040
     stem_loop       1588..1631
                     /label=stem_loop
     misc_feature    1577..1656
                     /label=BBa_B0010
     terminator      1577..1656
                     /label=BBa_B0010
     stem_loop       1672..1691
                     /label=T7 TE
     misc_feature    1698..1698
                     /label=stop
     polyA_site      1692..1705
                     /label=polya
     misc_feature    1665..1705
                     /label=BBa_B0012
     terminator      1665..1705
                     /label=BBa_B0012
     misc_feature    828..1705
                     /label=BBa_E0840
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agtacctgta ggatcgtaca ggtataaatt
      121 cttcagtact agagaaagag gagaaatact agatgatcgt tcagatcggt cgtcgtgaag
      181 agttcgacaa aaaactgctg ggtgaaatgc acaaactgcg tgctcaggtt ttcaaagaac
      241 gtaaaggttg ggacgtttcc gttatcgacg aaatggaaat cgacggttac gacgctctgt
      301 ccccgtacta catgctgatc caggaagaca ccccggaagc tcaggttttc ggttgctggc
      361 gtatcttcga caccaccggt ccgtacatgc tgaaaaacac cttcccggaa ctgctgcacg
      421 gtaaagaagc tccgtgctcc ccgcacatct gggaactgtc ccgtttcgct atcaactccg
      481 gtcagaaagg ttccctgggt ttctccgact gcaccctgga agctatgcgt gctctggctc
      541 gttactcctt gcagaacgac atccagaccc tggttaccgt taccaccgtt ggtgttgaaa
      601 aaatgatgat ccgtgctggt ctggacgttt cccgtttcgg tccgcacctg aaaatcggta
      661 tcgaacgtgc tgttgctctg cgtatcgaac tgaacgctaa aacccagatc gctctgtacg
      721 gtggtgttct ggttgaacag cgtctggctg tttccgctgc taacgacgaa aactacgctc
      781 tggttgctta ataactctga tagtgctagt gtagatctct actagagatt aaagaggaga
      841 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      901 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      961 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1021 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1081 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1141 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1201 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1261 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1321 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1381 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1441 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1501 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1561 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1621 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1681 tcgggtgggc ctttctgcgt ttata
//