LOCUS BBa_K266010 4256 bp DNA linear 22-Nov-2024 DEFINITION Tet & Lac constitutive Inverters controllin LasR & LuxR ACCESSION BBa_K266010 VERSION BBa_K266010.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 terminator 1223..1351 /label=BBa_B0015 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 44..1414 /label=BBa_Q04121 misc_feature 1427..1430 /label=conserved RBS 1423..1434 /label=BBa_B0034 CDS 1441..2157 /label=lasR misc_feature 2158..2190 /label=LVA misc_feature 1920..1920 /label=G misc_feature 2197..2221 /label=Help:Barcodes CDS 1441..2221 /label=BBa_C0079 stem_loop 2241..2284 /label=stem_loop misc_feature 2230..2309 /label=BBa_B0010 terminator 2230..2309 /label=BBa_B0010 stem_loop 2325..2344 /label=T7 TE misc_feature 2351..2351 /label=stop polyA_site 2345..2358 /label=polya misc_feature 2318..2358 /label=BBa_B0012 terminator 2318..2358 /label=BBa_B0012 terminator 2230..2358 /label=BBa_B0015 CDS 1441..2358 /label=BBa_K266002 misc_feature 44..2358 /label=BBa_K266003 misc_feature 1..2358 /label=BBa_K266004 promoter 2367..2401 /label=BBa_J23116 misc_feature 2414..2417 /label=conserved RBS 2410..2421 /label=BBa_B0034 CDS 2431..3047 /label=tetR misc_feature 3048..3081 /label=SsrA misc_feature 3088..3112 /label=Help:Barcodes CDS 2428..3112 /label=BBa_C0040 stem_loop 3132..3175 /label=stem_loop misc_feature 3121..3200 /label=BBa_B0010 terminator 3121..3200 /label=BBa_B0010 stem_loop 3216..3235 /label=T7 TE misc_feature 3242..3242 /label=stop polyA_site 3236..3249 /label=polya misc_feature 3209..3249 /label=BBa_B0012 terminator 3209..3249 /label=BBa_B0012 terminator 3121..3249 /label=BBa_B0015 misc_feature 2367..3249 /label=BBa_K145201 misc_feature 3258..3276 /label=TetR 1 misc_feature 3258..3311 /label=BBa_R0040 promoter 3277..3282 /label=-35 misc_feature 3283..3301 /label=TetR 2 promoter 3300..3305 /label=-10 promoter 3258..3311 /label=BBa_R0040 RBS 3320..3332 /label=RBS-3\Weak RBS 3326..3329 /label=RBS misc_feature 3320..3332 /label=BBa_B0032 RBS 3320..3332 /label=BBa_B0032 misc_feature 3339..3340 /label=prefix misc_feature 3512..3512 /label=T misc_feature 3830..3830 /label=A CDS 3339..4088 /label=luxR misc_feature 3339..4094 /label=BBa_C0062 misc_feature 4095..4119 /label=Help:Barcodes CDS 3339..4094 /label=BBa_C0062 misc_feature 3320..4119 /label=BBa_K116616 stem_loop 4139..4182 /label=stem_loop misc_feature 4128..4207 /label=BBa_B0010 terminator 4128..4207 /label=BBa_B0010 stem_loop 4223..4242 /label=T7 TE misc_feature 4249..4249 /label=stop polyA_site 4243..4256 /label=polya misc_feature 4216..4256 /label=BBa_B0012 terminator 4216..4256 /label=BBa_B0012 terminator 4128..4256 /label=BBa_B0015 misc_feature 3320..4256 /label=BBa_K116626 misc_feature 3258..4256 /label=BBa_K116640 misc_feature 2367..4256 /label=BBa_K266001 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag 1441 atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt ggagtggagc 1501 gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt cggcctgttg 1561 cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc ggccgcctgg 1621 cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag tcactgtacc 1681 cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa gcagcacgag 1741 ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc gctgcatggt 1801 gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg ggccgaggcc 1861 aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta cgcactgcaa 1921 agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct gaccagccgg 1981 gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat atcggttatc 2041 tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg gaagttcggt 2101 gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat tactctcgct 2161 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 2221 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2281 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2341 ggcctttctg cgtttatata ctagagttga cagctagctc agtcctaggg actatgctag 2401 ctactagaga aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac 2461 agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc 2521 cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg 2581 ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa 2641 ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta 2701 agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa 2761 actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta 2821 tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat 2881 caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga 2941 caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa 3001 ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac 3061 gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag 3121 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 3181 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 3241 gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc agtgatagag 3301 atactgagca ctactagagt cacacaggaa agtactagat gaaaaacata aatgccgacg 3361 acacatacag aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat 3421 gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc 3481 ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc 3541 aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca 3601 atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa 3661 atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata 3721 cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag 3781 atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata 3841 attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa 3901 aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag 3961 gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa 4021 caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact 4081 ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat 4141 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 4201 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //