##gff-version 3
##sequence-region BBa_K266010 1 4256
BBa_K266010	.	engineered_region	1	2358	.	+	0	ID=BBa_K266004;Name=BBa_K266004
BBa_K266010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K266004
BBa_K266010	.	engineered_region	44	2358	.	+	0	ID=BBa_K266003;Name=BBa_K266003;Parent=BBa_K266004
BBa_K266010	.	ribosome_entry_site	1423	1434	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K266003
BBa_K266010	.	conserved_region	1427	1430	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K266010	.	CDS	1441	2358	.	+	0	ID=BBa_K266002;Name=BBa_K266002;Parent=BBa_K266003
BBa_K266010	.	CDS	1441	2221	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_K266002
BBa_K266010	.	CDS	1441	2157	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K266010	.	tag	2158	2190	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K266010	.	engineered_tag	2197	2221	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K266010	.	sequence_alteration	1920	1920	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K266010	.	terminator	2230	2358	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K266002
BBa_K266010	.	terminator	2318	2358	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266010	.	stem_loop	2325	2344	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266010	.	stop_codon	2351	2351	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266010	.	polyA_site	2345	2358	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266010	.	engineered_region	2318	2358	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266010	.	terminator	2230	2309	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266010	.	stem_loop	2241	2284	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266010	.	engineered_region	2230	2309	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266010	.	engineered_region	44	1414	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_K266003
BBa_K266010	.	terminator	1223	1351	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_K266010	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266010	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266010	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266010	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266010	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266010	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266010	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266010	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266010	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_K266010	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K266010	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_K266010	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K266010	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K266010	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K266010	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K266010	.	promoter	1360	1413	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_K266010	.	promoter	1379	1384	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K266010	.	operator	1362	1378	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K266010	.	promoter	1402	1407	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K266010	.	engineered_region	1360	1413	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K266010	.	operator	1385	1401	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K266010	.	engineered_region	2367	4256	.	+	0	ID=BBa_K266001;Name=BBa_K266001
BBa_K266010	.	engineered_region	2367	3249	.	+	0	ID=BBa_K145201;Name=BBa_K145201;Parent=BBa_K266001
BBa_K266010	.	terminator	3121	3249	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K266010	.	terminator	3209	3249	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266010	.	stem_loop	3216	3235	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266010	.	stop_codon	3242	3242	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266010	.	polyA_site	3236	3249	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266010	.	engineered_region	3209	3249	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266010	.	terminator	3121	3200	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266010	.	stem_loop	3132	3175	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266010	.	engineered_region	3121	3200	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266010	.	ribosome_entry_site	2410	2421	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K266010	.	conserved_region	2414	2417	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K266010	.	CDS	2428	3112	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K266010	.	tag	3048	3081	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K266010	.	engineered_tag	3088	3112	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K266010	.	CDS	2431	3047	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K266010	.	promoter	2367	2401	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K266010	.	engineered_region	3258	4256	.	+	0	ID=BBa_K116640;Name=BBa_K116640;Parent=BBa_K266001
BBa_K266010	.	engineered_region	3320	4256	.	+	0	ID=BBa_K116626;Name=BBa_K116626;Parent=BBa_K116640
BBa_K266010	.	engineered_region	3320	4119	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116626
BBa_K266010	.	CDS	3339	4094	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K266010	.	engineered_region	3339	4094	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K266010	.	engineered_region	3339	3340	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K266010	.	point_mutation	3512	3512	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K266010	.	CDS	3339	4088	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K266010	.	point_mutation	3830	3830	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K266010	.	engineered_tag	4095	4119	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K266010	.	ribosome_entry_site	3320	3332	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K266010	.	engineered_region	3320	3332	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K266010	.	ribosome_entry_site	3326	3329	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K266010	.	ribosome_entry_site	3320	3332	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K266010	.	terminator	4128	4256	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116626
BBa_K266010	.	terminator	4216	4256	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K266010	.	stem_loop	4223	4242	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K266010	.	stop_codon	4249	4249	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K266010	.	polyA_site	4243	4256	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K266010	.	engineered_region	4216	4256	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K266010	.	terminator	4128	4207	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K266010	.	stem_loop	4139	4182	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K266010	.	engineered_region	4128	4207	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K266010	.	promoter	3258	3311	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K116640
BBa_K266010	.	engineered_region	3258	3311	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K266010	.	promoter	3300	3305	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K266010	.	promoter	3277	3282	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K266010	.	non_covalent_binding_site	3258	3276	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K266010	.	non_covalent_binding_site	3283	3301	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_K266010
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagag
aaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcg
ccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggacta
cgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgac
ccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacg
agttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcgg
cgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtgg
atgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgacca
gccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcgga
agccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggcc
gttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttga
cagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagta
aagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagct
aggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgtta
gataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagtttta
gatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactct
cgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcat
tttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagta
tgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaatt
gatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagct
taataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactaga
gtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtag
aagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatt
tatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacg
ctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaa
caatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccct
attcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttt
tacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataa
atcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttca
aaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgct
gccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata