LOCUS BBa_K268000 4814 bp DNA linear 15-Nov-2024 DEFINITION pSB6A0 ACCESSION BBa_K268000 VERSION BBa_K268000.1 FEATURES Location/Qualifiers CDS 817..1490 /label=TRP1 misc_feature 264..719 /label=F1_Ori, M13_Ori primer_bind 1837..1854 /label=M13F CDS 2870..3529 /label=AmpR misc_feature 1..21 /label=Biobrick suffix misc_feature 3627..4609 /label=ColE1_Origin misc_feature 4793..4814 /label=Biobrick prefix misc_feature 2022..2540 /label=CEN6_ARS4 ORIGIN 1 tactagtagc ggccgctgca gcccggggga tccaatagtt ctagtgcggc cgccaccgcg 61 gtggagctcc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca 121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 181 cttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 241 cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 301 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 361 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 421 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 481 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 541 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 601 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 661 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 721 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcag gcaagtgcac 781 aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa 841 tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca 901 ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct 961 aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga aatcgagttc 1021 caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga 1081 ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta 1141 ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg 1201 acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga 1261 aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt 1321 gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata 1381 cccagcaagt cagcatcgga atctagtgca cattctgcgg cctctgtgct ctgcaagccg 1441 caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct 1501 gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct 1561 tggccctctc cttttctttt ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc 1621 tccggatcaa gattgtacgt aaggtgacaa gctatttttc aataaagaat atcttccact 1681 actgccatct ggcgtcataa ctgcaaagta cacatatatt acgatgctgt ctattaaatg 1741 cttcctatat tatatatata gtaatgtcgt ttatggtgca ctctcagtac aatctgctct 1801 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 1861 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 1921 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 1981 ctatttttat aggttaatgt catgataata atggtttctt aggacggatc gcttgcctgt 2041 aacttacacg cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt 2101 atttattttt atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta 2161 cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta 2221 catatatatt tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa 2281 gtaaaatgta aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa 2341 ttcggcatta atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc 2401 cccctagagt cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt 2461 tactttctat ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat 2521 agcacgtgat gaaaaggacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat 2581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 2641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 2701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 2761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 2821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 2881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 2941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 3001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 3061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 3121 tcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 3181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 3241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 3301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 3361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 3421 tggtaagccc tcccgtatcg tagttatcta cacgacgggc agtcaggcaa ctatggatga 3481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 3541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 3601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 3661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 3721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 3781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 3841 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 3901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 3961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4081 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag gggggaacgc 4201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4261 atgctcgtca ggggggccga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4321 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 4381 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 4441 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 4501 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 4561 cagtgagcgc aacgcaatta atgtgagtta cctcactcat taggcacccc aggctttaca 4621 ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 4681 aaacagctat gaccatgatt acgccaagct cggaattaac cctcactaaa gggaacaaaa 4741 gctgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcgc 4801 ggccgcttct agag //