LOCUS BBa_K268016 2509 bp DNA linear 01-Nov-2024 DEFINITION YGR035C->GFP ACCESSION BBa_K268016 VERSION BBa_K268016.1 FEATURES Location/Qualifiers misc_feature 799..978 /label=TADH1 CDS 74..769 /label=GFPMut3 CDS 1405..2214 /label=KanR2 promoter 1061..1402 /label=PTEF promoter 1..40 /label=YGR035C_Promoter, forward primer region misc_feature 2223..2435 /label=TTEF_Terminator primer_bind 2472..2509 /label=YGR035C gene, reverse primer region ORIGIN 1 cggaagttat aagttatctt ctctacagaa acaatacgta atgcggatcc ccgggttaat 61 taacagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg 121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg 181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact 241 tgtcactact ttcacttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg 301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt 361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt 421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa 481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg 541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga 601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta 661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct 721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataggg 781 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt 841 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc 901 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc 961 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc 1021 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac 1081 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat 1141 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc 1201 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac 1261 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt 1321 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca 1381 catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga 1441 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg 1501 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg 1561 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg 1621 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca 1681 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct 1741 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt 1801 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca 1861 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct 1921 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc 1981 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt 2041 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac 2101 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat 2161 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagta 2221 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg 2281 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca 2341 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa 2401 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt taaacgagct 2461 cgaattccaa ccctgaggtc aatgtgctta actacttctt tgcatcttc //