LOCUS       BBa_K268016             2509 bp    DNA     linear       27-Dec-2024
DEFINITION  YGR035C->GFP
ACCESSION   BBa_K268016
VERSION     BBa_K268016.1  
FEATURES             Location/Qualifiers
     misc_feature    799..978
                     /label=TADH1
     CDS             74..769
                     /label=GFPMut3
     CDS             1405..2214
                     /label=KanR2
     promoter        1061..1402
                     /label=PTEF
     promoter        1..40
                     /label=YGR035C_Promoter, forward primer region
     misc_feature    2223..2435
                     /label=TTEF_Terminator
     primer_bind     2472..2509
                     /label=YGR035C gene, reverse primer region
ORIGIN
        1 cggaagttat aagttatctt ctctacagaa acaatacgta atgcggatcc ccgggttaat
       61 taacagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcacttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataggg
      781 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
      841 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
      901 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
      961 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc
     1021 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     1081 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     1141 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     1201 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
     1261 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     1321 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     1381 catcacatcc gaacataaac aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga
     1441 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1501 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
     1561 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1621 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1681 tggttactca ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct
     1741 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1801 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1861 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1921 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     1981 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2041 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2101 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2161 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagta
     2221 ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt ttttatattg
     2281 tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc ctcgacatca
     2341 tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa
     2401 tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt taaacgagct
     2461 cgaattccaa ccctgaggtc aatgtgctta actacttctt tgcatcttc
//