BBa_K270005 1 BBa_K270005 XylR 2009-10-18T11:00:00Z 2015-05-08T01:11:44Z NCBI: Pseudomonas putida plasmid pWW0 This is a regulatory protien that is under constituative expression of the Pr promoter. When no toluene is present it represses the Pr promoter and when toluene is present it binds with the toluene to activate the Pu promoter. false false _368_ 0 4465 9 Not in stock false This part was obtained using colony PCR of the entire Pr XylR region on the pWW0 plasmid. false Ann Lesnefsky annotation2054955 1 pWW0 range2054955 1 1702 1892 annotation2054954 1 XylR range2054954 1 1 1701 BBa_K270006 1 BBa_K270006 Pr Promoter 2009-10-18T11:00:00Z 2015-05-08T01:11:44Z NCBI:Pseudomonas putida plasmid pWW0 Pr is a regulatory promoter for the toluene degradation pathway on the pWW0 plasmid. This is a constituative promoter that expresses the regulatory protien XylR. When no toluene is present XylR represses Pr. When toluene is present the XylR binds with the toluene and the activity of the Pr promoter increases. false false _368_ 0 4465 9 Not in stock false The Pr XylR basic parts were amplified together from the pWW0 plasmid using colony PCR. false Ann Lesnefsky annotation2054959 1 UAS range2054959 1 284 300 annotation2054958 1 UAS range2054958 1 254 270 annotation2054956 1 pWW0 range2054956 1 1 221 annotation2054957 1 Pr Promoter range2054957 1 222 421 annotation2054960 1 IHF range2054960 1 252 273 BBa_K270001 1 BBa_K270001 Pr XylR 2009-10-18T11:00:00Z 2015-05-08T01:11:44Z NCBI: Pseudomonas putida plasmid pWW0 Pr XylR is found in Pseudomonas putida mt-2 on the pWW0-TOL plasmid which contains the metabolic pathway for degrading toluene. The regulation for the degration of toluene on this plasmid is shown in the figure below. [[Image:Toluene_degradation_topology.jpg|950px]] The image is adapted from Burlage, R. S., S. W. Hooper, and G. S. Sayler. 1989. The TOL (pWWO) catabolic plasmid. Appl. Environ. Microbiol. 55: 1323-1328. Toluene is degraded through two pathways: the upper and lower, meta-pathway. The upper pathway degrades toluene into catechol and the lower pathway degrades catechol into pyruvate and acetaldehyde. Pr is a constituative promoter that expresses the XylR regulator protien. When there is no toluene, XylR represses the Pr promoter and when there is toluene, XylR makes a complex with toluene that activates the Pu promoter expressing the upper pathway for toluene degradation. The XylR-toluene complex also relulates Ps and XylS, the regulatory promoter and protien for the lower pathway. This part is necessary for the regulation of the Pu promoter in species other then P. putida mt-2. We created this part to test Bba_XXXXXX, Pu GFP, in E. coli. This part can only be digested with EcoRI and SpeI to insert the part in front of the desired part because the XylR gene contains a PstI cutsite. false false _368_ 0 4465 9 It's complicated true From the Pseudomonas putida plasmid pWW0 NCBI sequence, an upper primer (5??? TATACTAGTCTGGGGCGAGAGGCGACGAC 3???)with an SpeI cutsite and a lower primer (5??? GGGGAATTCTAGAATGTGGGCTGCTTGGTG 3???) with EcoRI and XbaI cutsites were designed to amplify the 41246-43507bp region. false Ann Lesnefsky component2054978 1 BBa_K270005 component2054975 1 BBa_K270006 annotation2054975 1 BBa_K270006 range2054975 1 1 421 annotation2054978 1 BBa_K270005 range2054978 1 422 2313 BBa_K270006_sequence 1 tgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgataaaaacaagaggaaaacaa BBa_K270005_sequence 1 atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatagctgctttgcgccaggtttgggcctttctaaggtccttcgcggattctggggaaagagcgagttgacaggcaggggagtgggtaaattggcagtcgctcaacatcccaacctccacaccctgctgtcgccgtgcatcctattgatcttgccgctttctggccaggctacgacgtcgtcgcctctcgccccag BBa_K270001_sequence 1 tgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgataaaaacaagaggaaaacaaatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatagctgctttgcgccaggtttgggcctttctaaggtccttcgcggattctggggaaagagcgagttgacaggcaggggagtgggtaaattggcagtcgctcaacatcccaacctccacaccctgctgtcgccgtgcatcctattgatcttgccgctttctggccaggctacgacgtcgtcgcctctcgccccag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z