LOCUS       BBa_K270003             1226 bp    DNA     linear       26-Jun-2024
DEFINITION  Pu GFP
ACCESSION   BBa_K270003
VERSION     BBa_K270003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..137
                     /label=pWW0
     promoter        138..338
                     /label=Pu Promoter BBa_K270000
     misc_feature    165..214
                     /label=UAS
     misc_feature    268..283
                     /label=IHF
     misc_feature    339..340
                     /label=pWW0
     promoter        1..340
                     /label=BBa_K270000
     RBS             349..363
                     /label=RBS-1\Strong
     RBS             356..360
                     /label=RBS
     misc_feature    349..363
                     /label=BBa_B0030
     RBS             349..363
                     /label=BBa_B0030
     CDS             370..1089
                     /label=GFP protein
     CDS             370..1089
                     /label=BBa_E0040
     stem_loop       1109..1152
                     /label=stem_loop
     misc_feature    1098..1177
                     /label=BBa_B0010
     terminator      1098..1177
                     /label=BBa_B0010
     stem_loop       1193..1212
                     /label=T7 TE
     misc_feature    1219..1219
                     /label=stop
     polyA_site      1213..1226
                     /label=polya
     misc_feature    1186..1226
                     /label=BBa_B0012
     terminator      1186..1226
                     /label=BBa_B0012
ORIGIN
        1 cctgctggag ggcgtgaaca accgaatcaa ggtgatcaag cgcatggcct atggtttccg
       61 gggctcggac tacttcttcc tgaaagtcaa ggctgtcttc cccgggaaag cgcgatgaac
      121 cttttttatc gctgccttga tcaaatcgac aggtggttat gcgcgattga tgatttgctc
      181 aaatacagcc agcgtgctgt agattttctc tcataccccc cctttctttt ttacaaagaa
      241 aatcaataat ttagatgaaa taaggggatc ggtataagca atggcatggc ggttgctagc
      301 tatacgagac ttaaaataaa aatagtggtg acccttcaat tactagagat taaagaggag
      361 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      421 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      481 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      541 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      601 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      661 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      721 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      781 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      841 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      901 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      961 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1021 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1081 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1201 ttcgggtggg cctttctgcg tttata
//