LOCUS BBa_K270004 3553 bp DNA linear 27-Dec-2024 DEFINITION Pr XylR Pu GFP ACCESSION BBa_K270004 VERSION BBa_K270004.1 FEATURES Location/Qualifiers misc_feature 1..221 /label=pWW0 promoter 222..421 /label=Pr Promoter misc_feature 254..270 /label=UAS misc_feature 284..300 /label=UAS misc_feature 252..273 /label=IHF promoter 1..421 /label=BBa_K270006 CDS 428..2128 /label=XylR misc_feature 2129..2319 /label=pWW0 CDS 428..2319 /label=BBa_K270005 misc_feature 2328..2464 /label=pWW0 promoter 2465..2665 /label=Pu Promoter BBa_K270000 misc_feature 2492..2541 /label=UAS misc_feature 2595..2610 /label=IHF misc_feature 2666..2667 /label=pWW0 promoter 2328..2667 /label=BBa_K270000 RBS 2676..2690 /label=RBS-1\Strong RBS 2683..2687 /label=RBS misc_feature 2676..2690 /label=BBa_B0030 RBS 2676..2690 /label=BBa_B0030 CDS 2697..3416 /label=GFP protein CDS 2697..3416 /label=BBa_E0040 stem_loop 3436..3479 /label=stem_loop misc_feature 3425..3504 /label=BBa_B0010 terminator 3425..3504 /label=BBa_B0010 stem_loop 3520..3539 /label=T7 TE misc_feature 3546..3546 /label=stop polyA_site 3540..3553 /label=polya misc_feature 3513..3553 /label=BBa_B0012 terminator 3513..3553 /label=BBa_B0012 ORIGIN 1 tgggctgctt ggtgtgatgt agaaaggcgc caagtcgatg aaaatgcatc tcgacgtgat 61 gcgtatacgg gttaccccca ttgccacgtt gcgccatcct ttttgcaatc agtgaccact 121 tttccaagca aaaataacgc caagcagaac gaagacgttc tttttaagaa gcgagaacac 181 cagaagttcg tgctgtcggg gcatgcggcg acgaattggc ggataaaggg gatctgcgtt 241 gaggtggatt tcagttaatc aattggttaa tctttcagga ccacctaagc aaatgctaaa 301 gtggcagatg gaatgctgag ccggcaagca caggccttga cgttgcaagg tagtcatgac 361 cgcagtgagc ctctgatgtt ccgccgggtg gatcatcccg ataaaaacaa gaggaaaaca 421 atactagatg tcgcttacat acaaacccaa gatgcagcat gaggatatgc aagaccttag 481 cagccagatc cgtttcgttg ccgccgaagg caagatctgg ttgggagagc agcgcatgct 541 cgtaatgcag ctatctacgc tggccagctt ccgtcgcgaa attatcagct tgatcggcgt 601 cgagcgggcc aagggtttct tcctgcggtt gggctatcag tccggcctga tggatgccga 661 gctggcacgc aagctgcggc cggccatgcg cgaggaggag gtgttcctgg ctgggcctca 721 attgtatgcg ctcaagggga tggtcaaagt acgcttgctg acaatggata tcgccatccg 781 ggacggacgt ttcaacgtgg aggccgagtg gattgattcc tttgaagtgg atatctgccg 841 aactgagctg ggcctgatga atgagcccgt ctgctggacg gtgctaggct atgctagcgg 901 ctatggttcg gcattcatgg gccgcagaat cattttccag gaaactagct gtcgcgggtg 961 cggtgacgat aaatgcctta tcgtcggcaa gaccgcagaa gagtggggcg atgtcagcag 1021 tttcgaagcc tacttcaaaa gcgacccgat cgtagacgag cgctacgagc tgcagaccca 1081 ggttgccaac ctgcgcaacc gcctgaagca gtacgatggg cagtattacg gcattggcca 1141 ttcgccagcc tacaagcgca tctgtgagac catcgacaag gctgcacgcg gcagggtttc 1201 ggtcctgcta ctgggtgaga ctggggtggg caaggaggta atcgcgcgca gcgtgcattt 1261 gcgcagtgag cgcgcagagc aacccttcgt cgcggtgaac tgtgcggcaa ttccgccgga 1321 tctgatcgag tcggaactgt ttggtgtcga taagggcgcc tatacgggcg cggtcaatgc 1381 acgcgctgga cgttttgaac gggccaacgg cggcaccatc tttcttgatg aggtgatcga 1441 attgacgccg agggcccagg ccaccctgct acgggtattg caggaaggag agctagagcg 1501 ggtcggcggc gaccgcacgc gaaaggtcga cgtgaggtta atcaccgcaa caaacgagaa 1561 cctggaagag gcggtcaaga tggggcgctt tcgcgcagac ctgttctttc ggctgaatgt 1621 ttttcccgtg catatcccgc cgttgcgcga gcgcgtggaa gatatcccgc tgctggtcga 1681 gcattttctt agaaggcacc ataaggaata cggtaagaag actcttggcc tgtctgatcg 1741 agcgatggag gcctgcctcc actaccaatg gccaggcaat atccgcgagc tggagaacgc 1801 ccttgagcgc ggggtgattc ttaccgagag caacgaaagc atcaatgtcg agtcgctgtt 1861 cccggggttg gcgacggcta ccgaaggcga caggctatcg agcgagggcc ggttggagga 1921 ggagtccggt gacagttggt ttaggcaaat tatcgaccag ggcgtcagcc tcgaagatct 1981 cgaagcgggt ttaatgcgca cggccatgga ccgttgtggg cagaatatct cacaggcggc 2041 gcggttgctg ggattgaccc gcccggcaat ggcctatcga cttaagaagc ttgaccccag 2101 cttatctgtg aaagcaatgg gccgatagct gctttgcgcc aggtttgggc ctttctaagg 2161 tccttcgcgg attctgggga aagagcgagt tgacaggcag gggagtgggt aaattggcag 2221 tcgctcaaca tcccaacctc cacaccctgc tgtcgccgtg catcctattg atcttgccgc 2281 tttctggcca ggctacgacg tcgtcgcctc tcgccccagt actagagcct gctggagggc 2341 gtgaacaacc gaatcaaggt gatcaagcgc atggcctatg gtttccgggg ctcggactac 2401 ttcttcctga aagtcaaggc tgtcttcccc gggaaagcgc gatgaacctt ttttatcgct 2461 gccttgatca aatcgacagg tggttatgcg cgattgatga tttgctcaaa tacagccagc 2521 gtgctgtaga ttttctctca taccccccct ttctttttta caaagaaaat caataattta 2581 gatgaaataa ggggatcggt ataagcaatg gcatggcggt tgctagctat acgagactta 2641 aaataaaaat agtggtgacc cttcaattac tagagattaa agaggagaaa tactagatgc 2701 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 2761 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 2821 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 2881 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 2941 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 3001 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 3061 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 3121 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 3181 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 3241 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 3301 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 3361 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact 3421 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 3481 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 3541 ttctgcgttt ata //