The Pr XylR must come first because it has an illegal PstI cutsite
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
The Pu promoter is the upper pathway promoter for toluene degradation found on the TOL plasmid pWWO in P. putida mt-2.
The Pu promoter is not annotated on the NCBI sequence and transcribes the XylU, XylW, XylM, XylA, XylB and XylN genes. A 400 bp region between the XylU gene and the tnpA transposase was selected for amplification by colony PCR where the promoter was believed to reside.
This is a regulatory protien that is under constituative expression of the Pr promoter. When no toluene is present it represses the Pr promoter and when toluene is present it binds with the toluene to activate the Pu promoter.
This part was obtained using colony PCR of the entire Pr XylR region on the pWW0 plasmid.
Pr is a regulatory promoter for the toluene degradation pathway on the pWW0 plasmid. This is a constituative promoter that expresses the regulatory protien XylR. When no toluene is present XylR represses Pr. When toluene is present the XylR binds with the toluene and the activity of the Pr promoter increases.
The Pr XylR basic parts were amplified together from the pWW0 plasmid using colony PCR.
Conversion of the iGEM parts registry to SBOL2.1