BBa_K270004
type: ComponentDefinition
persistent identity: BBa K270004
display id: BBa_K270004
version: 1
was generated by: iGEM to SBOL conversion
description: Pr XylR Pu GFP
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:44Z
mutable provenance: See Bba_K270001 and Bba_K270003
owned by: james
owned by: myers
mutable description: This is a toluene sensor
discontinued: false
dominant: false
group u list: _368_
m user id: 0
owner id: 4465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The Pr XylR must come first because it has an illegal PstI cutsite
star: false
creator: Ann Lesnefsky
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K270000
component: BBa_K270005
component: BBa_E0040
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_K270006
sequence annotation: BBa_K270006
sequence annotation: BBa_K270005
sequence annotation: BBa_K270000
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K270004_sequence/1

BBa_K270000
type: Component
persistent identity: component2054993
display id: component2054993
version: 1
top level: BBa_K270004
definition: BBa_K270000
access: public

BBa_K270005
type: Component
persistent identity: component2054987
display id: component2054987
version: 1
top level: BBa_K270004
definition: BBa_K270005
access: public

BBa_E0040
type: Component
persistent identity: component2054998
display id: component2054998
version: 1
top level: BBa_K270004
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2054995
display id: component2054995
version: 1
top level: BBa_K270004
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component2055001
display id: component2055001
version: 1
top level: BBa_K270004
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2054999
display id: component2054999
version: 1
top level: BBa_K270004
definition: BBa_B0010
access: public

BBa_K270006
type: Component
persistent identity: component2054984
display id: component2054984
version: 1
top level: BBa_K270004
definition: BBa_K270006
access: public

BBa_K270006
type: SequenceAnnotation
persistent identity: annotation2054984
display id: annotation2054984
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2054984/range2054984/1
component: BBa_K270006

https://synbiohub.org/public/igem/BBa_K270004/annotation2054984/range2054984/1
type: Range
persistent identity: range2054984
display id: range2054984
version: 1
top level: BBa_K270004
direction: direction/reverse
start: 1
end: 421
orientation: inline

BBa_K270005
type: SequenceAnnotation
persistent identity: annotation2054987
display id: annotation2054987
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2054987/range2054987/1
component: BBa_K270005

https://synbiohub.org/public/igem/BBa_K270004/annotation2054987/range2054987/1
type: Range
persistent identity: range2054987
display id: range2054987
version: 1
top level: BBa_K270004
direction: direction/reverse
start: 428
end: 2319
orientation: inline

BBa_K270000
type: SequenceAnnotation
persistent identity: annotation2054993
display id: annotation2054993
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2054993/range2054993/1
component: BBa_K270000

https://synbiohub.org/public/igem/BBa_K270004/annotation2054993/range2054993/1
type: Range
persistent identity: range2054993
display id: range2054993
version: 1
top level: BBa_K270004
direction: direction/reverse
start: 2328
end: 2667
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2054995
display id: annotation2054995
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2054995/range2054995/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K270004/annotation2054995/range2054995/1
type: Range
persistent identity: range2054995
display id: range2054995
version: 1
top level: BBa_K270004
direction: direction/forward
start: 2676
end: 2690
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2054998
display id: annotation2054998
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2054998/range2054998/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K270004/annotation2054998/range2054998/1
type: Range
persistent identity: range2054998
display id: range2054998
version: 1
top level: BBa_K270004
direction: direction/reverse
start: 2697
end: 3416
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2054999
display id: annotation2054999
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2054999/range2054999/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K270004/annotation2054999/range2054999/1
type: Range
persistent identity: range2054999
display id: range2054999
version: 1
top level: BBa_K270004
direction: direction/forward
start: 3425
end: 3504
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055001
display id: annotation2055001
version: 1
top level: BBa_K270004
location: https://synbiohub.org/public/igem/BBa_K270004/annotation2055001/range2055001/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K270004/annotation2055001/range2055001/1
type: Range
persistent identity: range2055001
display id: range2055001
version: 1
top level: BBa_K270004
direction: direction/forward
start: 3513
end: 3553
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K270000
type: ComponentDefinition
persistent identity: BBa K270000
display id: BBa_K270000
version: 1
was generated by: iGEM to SBOL conversion
description: Pu Promoter
created: 2009-09-19T11:00:00Z
modified: 2015-05-08T01:11:44Z
mutable provenance: NCBI: Pseudomonas putida plasmid pWW0
owned by: james
owned by: myers
mutable description: The Pu promoter is the upper pathway promoter for toluene degradation found on the TOL plasmid pWWO in P. putida mt-2.
discontinued: false
dominant: false
experience: experience/None
group u list: _368_
m user id: 0
owner id: 4465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The Pu promoter is not annotated on the NCBI sequence and transcribes the XylU, XylW, XylM, XylA, XylB and XylN genes. A 400 bp region between the XylU gene and the tnpA transposase was selected for amplification by colony PCR where the promoter was believed to reside.
star: false
creator: Ann Lesnefsky
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pWW0
sequence annotation: Pu Promoter BBa_K270000
sequence annotation: UAS
sequence annotation: IHF
sequence: https://synbiohub.org/public/igem/BBa_K270000_sequence/1

pWW0
type: SequenceAnnotation
persistent identity: annotation2054889
display id: annotation2054889
version: 1
top level: BBa_K270000
location: https://synbiohub.org/public/igem/BBa_K270000/annotation2054889/range2054889/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K270000/annotation2054889/range2054889/1
type: Range
persistent identity: range2054889
display id: range2054889
version: 1
top level: BBa_K270000
direction: direction/forward
start: 1
end: 137
orientation: inline

Pu Promoter BBa_K270000
type: SequenceAnnotation
persistent identity: annotation2054890
display id: annotation2054890
version: 1
top level: BBa_K270000
location: https://synbiohub.org/public/igem/BBa_K270000/annotation2054890/range2054890/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K270000/annotation2054890/range2054890/1
type: Range
persistent identity: range2054890
display id: range2054890
version: 1
top level: BBa_K270000
direction: direction/forward
start: 138
end: 338
orientation: inline

UAS
type: SequenceAnnotation
persistent identity: annotation2054891
display id: annotation2054891
version: 1
top level: BBa_K270000
location: https://synbiohub.org/public/igem/BBa_K270000/annotation2054891/range2054891/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K270000/annotation2054891/range2054891/1
type: Range
persistent identity: range2054891
display id: range2054891
version: 1
top level: BBa_K270000
direction: direction/forward
start: 165
end: 214
orientation: inline

IHF
type: SequenceAnnotation
persistent identity: annotation2054892
display id: annotation2054892
version: 1
top level: BBa_K270000
location: https://synbiohub.org/public/igem/BBa_K270000/annotation2054892/range2054892/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K270000/annotation2054892/range2054892/1
type: Range
persistent identity: range2054892
display id: range2054892
version: 1
top level: BBa_K270000
direction: direction/forward
start: 268
end: 283
orientation: inline

pWW0
type: SequenceAnnotation
persistent identity: annotation2054893
display id: annotation2054893
version: 1
top level: BBa_K270000
location: https://synbiohub.org/public/igem/BBa_K270000/annotation2054893/range2054893/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K270000/annotation2054893/range2054893/1
type: Range
persistent identity: range2054893
display id: range2054893
version: 1
top level: BBa_K270000
direction: direction/forward
start: 339
end: 340
orientation: inline

BBa_K270005
type: ComponentDefinition
persistent identity: BBa K270005
display id: BBa_K270005
version: 1
was generated by: iGEM to SBOL conversion
description: XylR
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:44Z
mutable provenance: NCBI: Pseudomonas putida plasmid pWW0
owned by: james
owned by: myers
mutable description: This is a regulatory protien that is under constituative expression of the Pr promoter. When no toluene is present it represses the Pr promoter and when toluene is present it binds with the toluene to activate the Pu promoter.
discontinued: false
dominant: false
group u list: _368_
m user id: 0
owner id: 4465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was obtained using colony PCR of the entire Pr XylR region on the pWW0 plasmid.
star: false
creator: Ann Lesnefsky
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: XylR
sequence annotation: pWW0
sequence: https://synbiohub.org/public/igem/BBa_K270005_sequence/1

XylR
type: SequenceAnnotation
persistent identity: annotation2054954
display id: annotation2054954
version: 1
top level: BBa_K270005
location: https://synbiohub.org/public/igem/BBa_K270005/annotation2054954/range2054954/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K270005/annotation2054954/range2054954/1
type: Range
persistent identity: range2054954
display id: range2054954
version: 1
top level: BBa_K270005
direction: direction/forward
start: 1
end: 1701
orientation: inline

pWW0
type: SequenceAnnotation
persistent identity: annotation2054955
display id: annotation2054955
version: 1
top level: BBa_K270005
location: https://synbiohub.org/public/igem/BBa_K270005/annotation2054955/range2054955/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K270005/annotation2054955/range2054955/1
type: Range
persistent identity: range2054955
display id: range2054955
version: 1
top level: BBa_K270005
direction: direction/forward
start: 1702
end: 1892
orientation: inline

BBa_K270006
type: ComponentDefinition
persistent identity: BBa K270006
display id: BBa_K270006
version: 1
was generated by: iGEM to SBOL conversion
description: Pr Promoter
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:44Z
mutable provenance: NCBI:Pseudomonas putida plasmid pWW0
owned by: james
owned by: myers
mutable description: Pr is a regulatory promoter for the toluene degradation pathway on the pWW0 plasmid. This is a constituative promoter that expresses the regulatory protien XylR. When no toluene is present XylR represses Pr. When toluene is present the XylR binds with the toluene and the activity of the Pr promoter increases.
discontinued: false
dominant: false
group u list: _368_
m user id: 0
owner id: 4465
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The Pr XylR basic parts were amplified together from the pWW0 plasmid using colony PCR.
star: false
creator: Ann Lesnefsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pWW0
sequence annotation: Pr Promoter
sequence annotation: IHF
sequence annotation: UAS
sequence: https://synbiohub.org/public/igem/BBa_K270006_sequence/1

pWW0
type: SequenceAnnotation
persistent identity: annotation2054956
display id: annotation2054956
version: 1
top level: BBa_K270006
location: https://synbiohub.org/public/igem/BBa_K270006/annotation2054956/range2054956/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K270006/annotation2054956/range2054956/1
type: Range
persistent identity: range2054956
display id: range2054956
version: 1
top level: BBa_K270006
direction: direction/forward
start: 1
end: 221
orientation: inline

Pr Promoter
type: SequenceAnnotation
persistent identity: annotation2054957
display id: annotation2054957
version: 1
top level: BBa_K270006
location: https://synbiohub.org/public/igem/BBa_K270006/annotation2054957/range2054957/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K270006/annotation2054957/range2054957/1
type: Range
persistent identity: range2054957
display id: range2054957
version: 1
top level: BBa_K270006
direction: direction/forward
start: 222
end: 421
orientation: inline

IHF
type: SequenceAnnotation
persistent identity: annotation2054960
display id: annotation2054960
version: 1
top level: BBa_K270006
location: https://synbiohub.org/public/igem/BBa_K270006/annotation2054960/range2054960/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K270006/annotation2054960/range2054960/1
type: Range
persistent identity: range2054960
display id: range2054960
version: 1
top level: BBa_K270006
direction: direction/forward
start: 252
end: 273
orientation: inline

UAS
type: SequenceAnnotation
persistent identity: annotation2054958
display id: annotation2054958
version: 1
top level: BBa_K270006
location: https://synbiohub.org/public/igem/BBa_K270006/annotation2054958/range2054958/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K270006/annotation2054958/range2054958/1
type: Range
persistent identity: range2054958
display id: range2054958
version: 1
top level: BBa_K270006
direction: direction/forward
start: 254
end: 270
orientation: inline

UAS
type: SequenceAnnotation
persistent identity: annotation2054959
display id: annotation2054959
version: 1
top level: BBa_K270006
location: https://synbiohub.org/public/igem/BBa_K270006/annotation2054959/range2054959/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K270006/annotation2054959/range2054959/1
type: Range
persistent identity: range2054959
display id: range2054959
version: 1
top level: BBa_K270006
direction: direction/forward
start: 284
end: 300
orientation: inline

https://synbiohub.org/public/igem/BBa_K270004_sequence/1
type: Sequence
persistent identity: BBa K270004 sequence
display id: BBa_K270004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgataaaaacaagaggaaaacaatactagatgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K270000_sequence/1
type: Sequence
persistent identity: BBa K270000 sequence
display id: BBa_K270000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctgctggagggcgtgaacaaccgaatcaaggtgatcaagcgcatggcctatggtttccggggctcggactacttcttcctgaaagtcaaggctgtcttccccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K270005_sequence/1
type: Sequence
persistent identity: BBa K270005 sequence
display id: BBa_K270005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcgcttacatacaaacccaagatgcagcatgaggatatgcaagaccttagcagccagatccgtttcgttgccgccgaaggcaagatctggttgggagagcagcgcatgctcgtaatgcagctatctacgctggccagcttccgtcgcgaaattatcagcttgatcggcgtcgagcgggccaagggtttcttcctgcggttgggctatcagtccggcctgatggatgccgagctggcacgcaagctgcggccggccatgcgcgaggaggaggtgttcctggctgggcctcaattgtatgcgctcaaggggatggtcaaagtacgcttgctgacaatggatatcgccatccgggacggacgtttcaacgtggaggccgagtggattgattcctttgaagtggatatctgccgaactgagctgggcctgatgaatgagcccgtctgctggacggtgctaggctatgctagcggctatggttcggcattcatgggccgcagaatcattttccaggaaactagctgtcgcgggtgcggtgacgataaatgccttatcgtcggcaagaccgcagaagagtggggcgatgtcagcagtttcgaagcctacttcaaaagcgacccgatcgtagacgagcgctacgagctgcagacccaggttgccaacctgcgcaaccgcctgaagcagtacgatgggcagtattacggcattggccattcgccagcctacaagcgcatctgtgagaccatcgacaaggctgcacgcggcagggtttcggtcctgctactgggtgagactggggtgggcaaggaggtaatcgcgcgcagcgtgcatttgcgcagtgagcgcgcagagcaacccttcgtcgcggtgaactgtgcggcaattccgccggatctgatcgagtcggaactgtttggtgtcgataagggcgcctatacgggcgcggtcaatgcacgcgctggacgttttgaacgggccaacggcggcaccatctttcttgatgaggtgatcgaattgacgccgagggcccaggccaccctgctacgggtattgcaggaaggagagctagagcgggtcggcggcgaccgcacgcgaaaggtcgacgtgaggttaatcaccgcaacaaacgagaacctggaagaggcggtcaagatggggcgctttcgcgcagacctgttctttcggctgaatgtttttcccgtgcatatcccgccgttgcgcgagcgcgtggaagatatcccgctgctggtcgagcattttcttagaaggcaccataaggaatacggtaagaagactcttggcctgtctgatcgagcgatggaggcctgcctccactaccaatggccaggcaatatccgcgagctggagaacgcccttgagcgcggggtgattcttaccgagagcaacgaaagcatcaatgtcgagtcgctgttcccggggttggcgacggctaccgaaggcgacaggctatcgagcgagggccggttggaggaggagtccggtgacagttggtttaggcaaattatcgaccagggcgtcagcctcgaagatctcgaagcgggtttaatgcgcacggccatggaccgttgtgggcagaatatctcacaggcggcgcggttgctgggattgacccgcccggcaatggcctatcgacttaagaagcttgaccccagcttatctgtgaaagcaatgggccgatagctgctttgcgccaggtttgggcctttctaaggtccttcgcggattctggggaaagagcgagttgacaggcaggggagtgggtaaattggcagtcgctcaacatcccaacctccacaccctgctgtcgccgtgcatcctattgatcttgccgctttctggccaggctacgacgtcgtcgcctctcgccccag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K270006_sequence/1
type: Sequence
persistent identity: BBa K270006 sequence
display id: BBa_K270006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatgcggcgacgaattggcggataaaggggatctgcgttgaggtggatttcagttaatcaattggttaatctttcaggaccacctaagcaaatgctaaagtggcagatggaatgctgagccggcaagcacaggccttgacgttgcaaggtagtcatgaccgcagtgagcctctgatgttccgccgggtggatcatcccgataaaaacaagaggaaaacaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z