LOCUS       BBa_K272001              904 bp    DNA     linear       19-Dec-2024
DEFINITION  Constitutive Expression Cassette for RFP
ACCESSION   BBa_K272001
VERSION     BBa_K272001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..736
                     /label=mrfp1
     misc_feature    743..767
                     /label=Help:Barcodes
     CDS             62..742
                     /label=BBa_E1010
     stem_loop       787..830
                     /label=stem_loop
     misc_feature    776..855
                     /label=BBa_B0010
     terminator      776..855
                     /label=BBa_B0010
     stem_loop       871..890
                     /label=T7 TE
     misc_feature    897..897
                     /label=stop
     polyA_site      891..904
                     /label=polya
     misc_feature    864..904
                     /label=BBa_B0012
     terminator      864..904
                     /label=BBa_B0012
     misc_feature    44..904
                     /label=BBa_I13507
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
      361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
      421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
      481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
      541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
      601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
      661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
      721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      901 tata
//