LOCUS       BBa_K272500             2412 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive expression cassette for TetR repressor-mRFP-Spectinomy
            cin resistance marker
ACCESSION   BBa_K272500
VERSION     BBa_K272500.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     misc_feature    44..746
                     /label=BBa_S03879
     misc_feature    759..762
                     /label=conserved
     RBS             755..766
                     /label=BBa_B0034
     CDS             773..1447
                     /label=mrfp1
     misc_feature    1454..1478
                     /label=Help:Barcodes
     CDS             773..1453
                     /label=BBa_E1010
     misc_feature    755..1478
                     /label=BBa_I13502
     misc_feature    1491..1494
                     /label=conserved
     RBS             1487..1498
                     /label=BBa_B0034
     CDS             1505..2269
                     /label=Aad9 Spectinomycin Acetyltransferase
     misc_feature    2270..2272
                     /label=stop
     misc_feature    2273..2275
                     /label=stop
     misc_feature    1505..1507
                     /label=start
     CDS             1505..2275
                     /label=BBa_K143031
     stem_loop       2295..2338
                     /label=stem_loop
     misc_feature    2284..2363
                     /label=BBa_B0010
     terminator      2284..2363
                     /label=BBa_B0010
     stem_loop       2379..2398
                     /label=T7 TE
     misc_feature    2405..2405
                     /label=stop
     polyA_site      2399..2412
                     /label=polya
     misc_feature    2372..2412
                     /label=BBa_B0012
     terminator      2372..2412
                     /label=BBa_B0012
     misc_feature    1505..2412
                     /label=BBa_K143065
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatggcttc
      781 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      841 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      901 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      961 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1021 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1081 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1141 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1201 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1261 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     1321 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     1381 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     1441 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagaaag aggagaaata
     1501 ctagatgagg aggatatatt tgaatacata cgaacaaatt aataaagtga aaaaaatact
     1561 tcggaaacat ttaaaaaata accttattgg tacttacatg tttggatcag gagttgagag
     1621 tggactaaaa ccaaatagtg atcttgactt tttagtcgtc gtatctgaac cattgacaga
     1681 tcaaagtaaa gaaatactta tacaaaaaat tagacctatt tcaaaaaaaa taggagataa
     1741 aagcaactta cgatatattg aattaacaat tattattcag caagaaatgg taccgtggaa
     1801 tcatcctccc aaacaagaat ttatttatgg agaatggtta caagagcttt atgaacaagg
     1861 atacattcct cagaaggaat taaattcaga tttaaccata atgctttacc aagcaaaacg
     1921 aaaaaataaa agaatatacg gaaattatga cttagaggaa ttactacctg atattccatt
     1981 ttctgatgtg agaagagcca ttatggattc gtcagaggaa ttaatagata attatcagga
     2041 tgatgaaacc aactctatat taactttatg ccgtatgatt ttaactatgg acacgggtaa
     2101 aatcatacca aaagatattg cgggaaatgc agtggctgaa tcttctccat tagaacatag
     2161 ggagagaatt ttgttagcag ttcgtagtta tcttggagag aatattgaat ggactaatga
     2221 aaatgtaaat ttaactataa actatttaaa taacagatta aaaaaattat aataatacta
     2281 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2341 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2401 tctgcgttta ta
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