##gff-version 3
##sequence-region BBa_K272500 1 2412
BBa_K272500	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K272500	.	ribosome_entry_site	1487	1498	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K272500	.	conserved_region	1491	1494	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272500	.	engineered_region	755	1478	.	+	0	ID=BBa_I13502;Name=BBa_I13502
BBa_K272500	.	ribosome_entry_site	755	766	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_K272500	.	conserved_region	759	762	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272500	.	CDS	773	1453	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_K272500	.	engineered_tag	1454	1478	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K272500	.	CDS	773	1447	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K272500	.	engineered_region	44	746	.	+	0	ID=BBa_S03879;Name=BBa_S03879
BBa_K272500	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03879
BBa_K272500	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K272500	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K272500	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K272500	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03879
BBa_K272500	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272500	.	engineered_region	1505	2412	.	+	0	ID=BBa_K143065;Name=BBa_K143065
BBa_K272500	.	terminator	2372	2412	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143065
BBa_K272500	.	stem_loop	2379	2398	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K272500	.	stop_codon	2405	2405	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K272500	.	polyA_site	2399	2412	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K272500	.	engineered_region	2372	2412	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K272500	.	terminator	2284	2363	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143065
BBa_K272500	.	stem_loop	2295	2338	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K272500	.	engineered_region	2284	2363	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K272500	.	CDS	1505	2275	.	+	0	ID=BBa_K143031;Name=BBa_K143031;Parent=BBa_K143065
BBa_K272500	.	start_codon	1505	1507	.	+	0	ID=annotation1992698;Name=start;Parent=BBa_K143031
BBa_K272500	.	stop_codon	2270	2272	.	+	0	ID=annotation1992696;Name=stop;Parent=BBa_K143031
BBa_K272500	.	stop_codon	2273	2275	.	+	0	ID=annotation1992697;Name=stop;Parent=BBa_K143031
BBa_K272500	.	CDS	1505	2269	.	+	0	ID=annotation1975961;Name=Aad9 Spectinomycin Acetyltransferase;Parent=BBa_K143031
>BBa_K272500
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggcttcctccgaagac
gttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaag
gtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggga
catcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactg
tccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcct
ccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaa
aaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgt
ctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgg
gtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgc
tgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatac
tagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaata
accttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgt
cgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagat
aaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaag
aatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaac
cataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatatt
ccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaact
ctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagt
ggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatgg
actaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata