LOCUS BBa_K272501 3016 bp DNA linear 22-Dec-2024 DEFINITION TetR repressible promoter-Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance ma ACCESSION BBa_K272501 VERSION BBa_K272501.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..83 /label=start misc_feature 384..386 /label=stop CDS 81..383 /label=cds CDS 81..386 /label=BBa_K145151 misc_feature 399..402 /label=conserved RBS 395..406 /label=BBa_B0034 CDS 413..1135 /label=BBa_E0030 stem_loop 1155..1198 /label=stem_loop misc_feature 1144..1223 /label=BBa_B0010 terminator 1144..1223 /label=BBa_B0010 stem_loop 1239..1258 /label=T7 TE misc_feature 1265..1265 /label=stop polyA_site 1259..1272 /label=polya misc_feature 1232..1272 /label=BBa_B0012 terminator 1232..1272 /label=BBa_B0012 promoter 1281..1315 /label=BBa_J23119 misc_feature 1328..1331 /label=conserved RBS 1324..1335 /label=BBa_B0034 CDS 1342..2130 /label=BBa_K156011 misc_feature 2143..2146 /label=conserved RBS 2139..2150 /label=BBa_B0034 CDS 2157..2879 /label=BBa_E0020 stem_loop 2899..2942 /label=stem_loop misc_feature 2888..2967 /label=BBa_B0010 terminator 2888..2967 /label=BBa_B0010 stem_loop 2983..3002 /label=T7 TE misc_feature 3009..3009 /label=stop polyA_site 3003..3016 /label=polya misc_feature 2976..3016 /label=BBa_B0012 terminator 2976..3016 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcagttta aggtttacac ctataaaaga gagagccgtt 121 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 181 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 241 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 301 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 361 ttaacctgat gttctgggga atataatact agagaaagag gagaaatact agatggtgag 421 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 481 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 541 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 601 caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga agctgcacga 661 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 721 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 781 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 841 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 901 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 961 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 1021 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 1081 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta 1141 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 1201 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 1261 tctgcgttta tatactagag ttgacagcta gctcagtcct aggtataatg ctagctacta 1321 gagaaagagg agaaatacta gatgcgcgaa gcggttattg ctgaagtttc tactcaactg 1381 tctgaagttg tgggtgtaat cgaacgccac ctggagccaa ccctgctggc tgtccacctg 1441 tacggctctg ctgtggacgg tggtctgaaa ccacattccg acattgacct gctggtaact 1501 gtgaccgtgc gtctggacga aactacccgt cgcgcgctga ttaacgacct gctggaaact 1561 tctgcttctc cgggtgaatc cgaaatcctg cgtgcggtag aggttacgat tgtggttcat 1621 gacgatatta tcccgtggcg ttaccctgct aagcgtgaac tgcaatttgg tgagtggcag 1681 cgcaacgaca ttctggcagg tatctttgaa ccggcgacca tcgacatcga cctggctatc 1741 ctgctgacta aagcgcgtga gcacagcgta gcgctggtgg gccctgctgc cgaggaactg 1801 ttcgacccgg ttccggaaca agatctgttc gaagctctga acgaaactct gaccctgtgg 1861 aactctccgc cggactgggc aggtgatgaa cgcaacgtag ttctgactct gtcccgcatt 1921 tggtattccg ctgtgaccgg caaaattgcc cctaaagacg tcgctgcgga ttgggctatg 1981 gaacgtctgc ctgctcaata tcagccggtg atcctggaag cacgtcaggc gtacctgggc 2041 caggaggacc gcctggcaag ccgtgctgac cagctggaag agttcgtaca ctacgttaag 2101 ggcgagatca ctaaggtagt gggtaagtga tactagagaa agaggagaaa tactagatgg 2161 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 2221 acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 2281 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 2341 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 2401 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 2461 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 2521 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 2581 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca 2641 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 2701 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 2761 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 2821 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat 2881 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2941 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 3001 cctttctgcg tttata //