##gff-version 3
##sequence-region BBa_K272501 1 3016
BBa_K272501	.	terminator	1232	1272	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K272501	.	engineered_region	1232	1272	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K272501	.	stop_codon	1265	1265	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K272501	.	stem_loop	1239	1258	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K272501	.	polyA_site	1259	1272	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K272501	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K272501	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K272501	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K272501	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K272501	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K272501	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K272501	.	CDS	81	386	.	+	0	ID=BBa_K145151;Name=BBa_K145151
BBa_K272501	.	CDS	81	383	.	+	0	ID=annotation1970253;Name=cds;Parent=BBa_K145151
BBa_K272501	.	stop_codon	384	386	.	+	0	ID=annotation1970252;Name=stop;Parent=BBa_K145151
BBa_K272501	.	start_codon	81	83	.	+	0	ID=annotation1970251;Name=start;Parent=BBa_K145151
BBa_K272501	.	terminator	2888	2967	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K272501	.	engineered_region	2888	2967	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K272501	.	stem_loop	2899	2942	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K272501	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K272501	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272501	.	ribosome_entry_site	2139	2150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K272501	.	conserved_region	2143	2146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272501	.	ribosome_entry_site	1324	1335	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K272501	.	conserved_region	1328	1331	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272501	.	promoter	1281	1315	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K272501	.	CDS	2157	2879	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_K272501	.	ribosome_entry_site	395	406	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K272501	.	conserved_region	399	402	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272501	.	terminator	1144	1223	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K272501	.	engineered_region	1144	1223	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K272501	.	stem_loop	1155	1198	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K272501	.	terminator	2976	3016	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K272501	.	engineered_region	2976	3016	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K272501	.	stop_codon	3009	3009	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K272501	.	stem_loop	2983	3002	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K272501	.	polyA_site	3003	3016	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K272501	.	CDS	413	1135	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_K272501	.	CDS	1342	2130	.	+	0	ID=BBa_K156011;Name=BBa_K156011
>BBa_K272501
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgac
acgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccgg
tggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaaga
agtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagaga
aagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacgg
cgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttc
atctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgccc
gctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatctt
cttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctg
aagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctata
tcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagct
cgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccag
tccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactc
tcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagat
gcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaacc
ctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactg
tgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatc
cgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaa
ctgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggcta
tcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccgga
acaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaac
gtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattggg
ctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcct
ggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtgatac
tagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagct
ggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctg
aagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgct
tcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcac
catcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatc
gagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacg
tctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgt
gcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagc
acccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggga
tcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata