BBa_K272501
type: ComponentDefinition
persistent identity: BBa K272501
display id: BBa_K272501
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter-Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance ma
created: 2009-10-20T11:00:00Z
modified: 2015-07-09T01:04:39Z
mutable provenance: Registry of Standard Biological Parts
owned by: james
owned by: myers
mutable description: TetR repressible promoter-Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance marker-ECFP
discontinued: true
dominant: false
experience: experience/None
group u list: _369_
m user id: 4206
owner id: 5014
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: Standard Assembly
star: false
creator: IIT_Madras 2009
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_K145151
component: BBa_B0010
component: BBa_E0030
component: BBa_R0040
component: BBa_E0020
component: BBa_B0012
component: BBa_J23119
component: BBa_K156011
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K145151
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23119
sequence annotation: BBa_K156011
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_K272501_sequence/1

BBa_B0034
type: Component
persistent identity: component2057906
display id: component2057906
version: 1
top level: BBa_K272501
definition: BBa_B0034
access: public

BBa_K145151
type: Component
persistent identity: component2057904
display id: component2057904
version: 1
top level: BBa_K272501
definition: ccdB
access: public

BBa_B0010
type: Component
persistent identity: component2057908
display id: component2057908
version: 1
top level: BBa_K272501
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component2057907
display id: component2057907
version: 1
top level: BBa_K272501
definition: eyfp
access: public

BBa_B0034
type: Component
persistent identity: component2057900
display id: component2057900
version: 1
top level: BBa_K272501
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2057894
display id: component2057894
version: 1
top level: BBa_K272501
definition: p(tetR)
access: public

BBa_E0020
type: Component
persistent identity: component2057920
display id: component2057920
version: 1
top level: BBa_K272501
definition: ecfp
access: public

BBa_B0034
type: Component
persistent identity: component2057919
display id: component2057919
version: 1
top level: BBa_K272501
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2057923
display id: component2057923
version: 1
top level: BBa_K272501
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2057921
display id: component2057921
version: 1
top level: BBa_K272501
definition: BBa_B0010
access: public

BBa_J23119
type: Component
persistent identity: component2057914
display id: component2057914
version: 1
top level: BBa_K272501
definition: BBa_J23119
access: public

BBa_B0012
type: Component
persistent identity: component2057910
display id: component2057910
version: 1
top level: BBa_K272501
definition: BBa_B0012
access: public

BBa_K156011
type: Component
persistent identity: component2057917
display id: component2057917
version: 1
top level: BBa_K272501
definition: BBa_K156011
access: public

BBa_B0034
type: Component
persistent identity: component2057916
display id: component2057916
version: 1
top level: BBa_K272501
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2057894
display id: annotation2057894
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057894/range2057894/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K272501/annotation2057894/range2057894/1
type: Range
persistent identity: range2057894
display id: range2057894
version: 1
top level: BBa_K272501
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2057900
display id: annotation2057900
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057900/range2057900/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K272501/annotation2057900/range2057900/1
type: Range
persistent identity: range2057900
display id: range2057900
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K145151
type: SequenceAnnotation
persistent identity: annotation2057904
display id: annotation2057904
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057904/range2057904/1
component: BBa_K145151

https://synbiohub.org/public/igem/BBa_K272501/annotation2057904/range2057904/1
type: Range
persistent identity: range2057904
display id: range2057904
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 81
end: 386
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2057906
display id: annotation2057906
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057906/range2057906/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K272501/annotation2057906/range2057906/1
type: Range
persistent identity: range2057906
display id: range2057906
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 395
end: 406
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2057907
display id: annotation2057907
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057907/range2057907/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K272501/annotation2057907/range2057907/1
type: Range
persistent identity: range2057907
display id: range2057907
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 413
end: 1135
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2057908
display id: annotation2057908
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057908/range2057908/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K272501/annotation2057908/range2057908/1
type: Range
persistent identity: range2057908
display id: range2057908
version: 1
top level: BBa_K272501
direction: direction/forward
start: 1144
end: 1223
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2057910
display id: annotation2057910
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057910/range2057910/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K272501/annotation2057910/range2057910/1
type: Range
persistent identity: range2057910
display id: range2057910
version: 1
top level: BBa_K272501
direction: direction/forward
start: 1232
end: 1272
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2057914
display id: annotation2057914
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057914/range2057914/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K272501/annotation2057914/range2057914/1
type: Range
persistent identity: range2057914
display id: range2057914
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 1281
end: 1315
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2057916
display id: annotation2057916
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057916/range2057916/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K272501/annotation2057916/range2057916/1
type: Range
persistent identity: range2057916
display id: range2057916
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 1324
end: 1335
orientation: inline

BBa_K156011
type: SequenceAnnotation
persistent identity: annotation2057917
display id: annotation2057917
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057917/range2057917/1
component: BBa_K156011

https://synbiohub.org/public/igem/BBa_K272501/annotation2057917/range2057917/1
type: Range
persistent identity: range2057917
display id: range2057917
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 1342
end: 2130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2057919
display id: annotation2057919
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057919/range2057919/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K272501/annotation2057919/range2057919/1
type: Range
persistent identity: range2057919
display id: range2057919
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 2139
end: 2150
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation2057920
display id: annotation2057920
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057920/range2057920/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_K272501/annotation2057920/range2057920/1
type: Range
persistent identity: range2057920
display id: range2057920
version: 1
top level: BBa_K272501
direction: direction/reverse
start: 2157
end: 2879
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2057921
display id: annotation2057921
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057921/range2057921/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K272501/annotation2057921/range2057921/1
type: Range
persistent identity: range2057921
display id: range2057921
version: 1
top level: BBa_K272501
direction: direction/forward
start: 2888
end: 2967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2057923
display id: annotation2057923
version: 1
top level: BBa_K272501
location: https://synbiohub.org/public/igem/BBa_K272501/annotation2057923/range2057923/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K272501/annotation2057923/range2057923/1
type: Range
persistent identity: range2057923
display id: range2057923
version: 1
top level: BBa_K272501
direction: direction/forward
start: 2976
end: 3016
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa K145151
display id: BBa_K145151
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB coding region
created: 2008-08-06T11:00:00Z
modified: 2015-07-08T03:14:50Z
mutable provenance: P1010
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the ccdB (control of cell death) gene.
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 4206
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable notes: just one stop codon in the end
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145151_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1970251
display id: annotation1970251
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
type: Range
persistent identity: range1970251
display id: range1970251
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1970253
display id: annotation1970253
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
type: Range
persistent identity: range1970253
display id: range1970253
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 303
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1970252
display id: annotation1970252
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
type: Range
persistent identity: range1970252
display id: range1970252
version: 1
top level: ccdB
direction: direction/forward
start: 304
end: 306
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K156011
type: ComponentDefinition
persistent identity: BBa K156011
display id: BBa_K156011
version: 1
was generated by: iGEM to SBOL conversion
description: aadA (streptomycin 3'-adenyltransferase)
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:10:54Z
mutable provenance: aadA was isolated from a plasmid in ''E. coli''. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438).
owned by: james
owned by: myers
mutable description: This part codes for an aminoglycoside resistance protein that endows ''E. coli'' with resistance to the antibiotic streptomycin. This part can be used as a selection marker.
discontinued: false
dominant: false
experience: experience/None
group u list: _246_
m user id: 0
owner id: 1475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
star: true
creator: George McArthur IV
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K156011_sequence/1

https://synbiohub.org/public/igem/BBa_K272501_sequence/1
type: Sequence
persistent identity: BBa K272501 sequence
display id: BBa_K272501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145151_sequence/1
type: Sequence
persistent identity: BBa K145151 sequence
display id: BBa_K145151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K156011_sequence/1
type: Sequence
persistent identity: BBa K156011 sequence
display id: BBa_K156011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcctggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z