##gff-version 3
##sequence-region BBa_K272502 1 1615
BBa_K272502	.	engineered_region	44	746	.	+	0	ID=BBa_S03879;Name=BBa_S03879
BBa_K272502	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S03879
BBa_K272502	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K272502	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K272502	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K272502	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03879
BBa_K272502	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272502	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K272502	.	engineered_region	755	1615	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K272502	.	ribosome_entry_site	755	766	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K272502	.	conserved_region	759	762	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K272502	.	terminator	1487	1566	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K272502	.	stem_loop	1498	1541	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K272502	.	engineered_region	1487	1566	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K272502	.	terminator	1575	1615	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K272502	.	engineered_region	1575	1615	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K272502	.	stop_codon	1608	1608	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K272502	.	polyA_site	1602	1615	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K272502	.	stem_loop	1582	1601	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K272502	.	CDS	773	1453	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K272502	.	CDS	773	1447	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K272502	.	engineered_tag	1454	1478	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K272502
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggcttcctccgaagac
gttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaag
gtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggga
catcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactg
tccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcct
ccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaa
aaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgt
ctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgg
gtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgc
tgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata