LOCUS       BBa_K272990             1572 bp    DNA     linear       22-Dec-2024
DEFINITION  Rbs-Tet repressor-mRFP
ACCESSION   BBa_K272990
VERSION     BBa_K272990.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..703
                     /label=BBa_C0040
     misc_feature    1..703
                     /label=BBa_S03879
     misc_feature    716..719
                     /label=conserved
     RBS             712..723
                     /label=BBa_B0034
     CDS             730..1404
                     /label=mrfp1
     misc_feature    1411..1435
                     /label=Help:Barcodes
     CDS             730..1410
                     /label=BBa_E1010
     stem_loop       1455..1498
                     /label=stem_loop
     misc_feature    1444..1523
                     /label=BBa_B0010
     terminator      1444..1523
                     /label=BBa_B0010
     stem_loop       1539..1558
                     /label=T7 TE
     misc_feature    1565..1565
                     /label=stop
     polyA_site      1559..1572
                     /label=polya
     misc_feature    1532..1572
                     /label=BBa_B0012
     terminator      1532..1572
                     /label=BBa_B0012
     misc_feature    712..1572
                     /label=BBa_I13507
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag
      721 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      781 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
      841 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
      901 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
      961 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
     1021 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
     1081 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
     1141 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
     1201 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
     1261 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
     1321 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
     1381 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta
     1441 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1501 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1561 tctgcgttta ta
//