LOCUS BBa_K272990 1572 bp DNA linear 31-Oct-2024 DEFINITION Rbs-Tet repressor-mRFP ACCESSION BBa_K272990 VERSION BBa_K272990.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..638 /label=tetR misc_feature 639..672 /label=SsrA misc_feature 679..703 /label=Help:Barcodes CDS 19..703 /label=BBa_C0040 misc_feature 1..703 /label=BBa_S03879 misc_feature 716..719 /label=conserved RBS 712..723 /label=BBa_B0034 CDS 730..1404 /label=mrfp1 misc_feature 1411..1435 /label=Help:Barcodes CDS 730..1410 /label=BBa_E1010 stem_loop 1455..1498 /label=stem_loop misc_feature 1444..1523 /label=BBa_B0010 terminator 1444..1523 /label=BBa_B0010 stem_loop 1539..1558 /label=T7 TE misc_feature 1565..1565 /label=stop polyA_site 1559..1572 /label=polya misc_feature 1532..1572 /label=BBa_B0012 terminator 1532..1572 /label=BBa_B0012 misc_feature 712..1572 /label=BBa_I13507 ORIGIN 1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag 721 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt 781 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg 841 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct 901 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct 961 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg 1021 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag 1081 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag 1141 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg 1201 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt 1261 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc 1321 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct 1381 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta 1441 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 1501 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 1561 tctgcgttta ta //