LOCUS BBa_K272991 2954 bp DNA linear 01-Jun-2024 DEFINITION Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance marker-ECFP ACCESSION BBa_K272991 VERSION BBa_K272991.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..21 /label=start misc_feature 322..324 /label=stop CDS 19..321 /label=cds CDS 19..324 /label=BBa_K145151 misc_feature 337..340 /label=conserved RBS 333..344 /label=BBa_B0034 CDS 351..1073 /label=BBa_E0030 stem_loop 1093..1136 /label=stem_loop misc_feature 1082..1161 /label=BBa_B0010 terminator 1082..1161 /label=BBa_B0010 stem_loop 1177..1196 /label=T7 TE misc_feature 1203..1203 /label=stop polyA_site 1197..1210 /label=polya misc_feature 1170..1210 /label=BBa_B0012 terminator 1170..1210 /label=BBa_B0012 promoter 1219..1253 /label=BBa_J23119 misc_feature 1266..1269 /label=conserved RBS 1262..1273 /label=BBa_B0034 CDS 1280..2068 /label=BBa_K156011 misc_feature 2081..2084 /label=conserved RBS 2077..2088 /label=BBa_B0034 CDS 2095..2817 /label=BBa_E0020 stem_loop 2837..2880 /label=stem_loop misc_feature 2826..2905 /label=BBa_B0010 terminator 2826..2905 /label=BBa_B0010 stem_loop 2921..2940 /label=T7 TE misc_feature 2947..2947 /label=stop polyA_site 2941..2954 /label=polya misc_feature 2914..2954 /label=BBa_B0012 terminator 2914..2954 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gcagtttaag gtttacacct ataaaagaga gagccgttat 61 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc 121 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg 181 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc 241 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt 301 aacctgatgt tctggggaat ataatactag agaaagagga gaaatactag atggtgagca 361 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 421 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 481 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 541 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact 601 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 661 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 721 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 781 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 841 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 901 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct 961 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 1021 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga 1081 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1141 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1201 tgcgtttata tactagagtt gacagctagc tcagtcctag gtataatgct agctactaga 1261 gaaagaggag aaatactaga tgcgcgaagc ggttattgct gaagtttcta ctcaactgtc 1321 tgaagttgtg ggtgtaatcg aacgccacct ggagccaacc ctgctggctg tccacctgta 1381 cggctctgct gtggacggtg gtctgaaacc acattccgac attgacctgc tggtaactgt 1441 gaccgtgcgt ctggacgaaa ctacccgtcg cgcgctgatt aacgacctgc tggaaacttc 1501 tgcttctccg ggtgaatccg aaatcctgcg tgcggtagag gttacgattg tggttcatga 1561 cgatattatc ccgtggcgtt accctgctaa gcgtgaactg caatttggtg agtggcagcg 1621 caacgacatt ctggcaggta tctttgaacc ggcgaccatc gacatcgacc tggctatcct 1681 gctgactaaa gcgcgtgagc acagcgtagc gctggtgggc cctgctgccg aggaactgtt 1741 cgacccggtt ccggaacaag atctgttcga agctctgaac gaaactctga ccctgtggaa 1801 ctctccgccg gactgggcag gtgatgaacg caacgtagtt ctgactctgt cccgcatttg 1861 gtattccgct gtgaccggca aaattgcccc taaagacgtc gctgcggatt gggctatgga 1921 acgtctgcct gctcaatatc agccggtgat cctggaagca cgtcaggcgt acctgggcca 1981 ggaggaccgc ctggcaagcc gtgctgacca gctggaagag ttcgtacact acgttaaggg 2041 cgagatcact aaggtagtgg gtaagtgata ctagagaaag aggagaaata ctagatggtg 2101 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2161 gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2221 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 2281 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 2341 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 2401 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 2461 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 2521 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 2581 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 2641 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 2701 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 2761 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac 2821 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2881 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2941 tttctgcgtt tata //