BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K145151 1 ccdB ccdB coding region 2008-08-06T11:00:00Z 2015-07-08T03:14:50Z P1010 Released HQ 2013 Coding region for the ccdB (control of cell death) gene. true false _257_ 4206 2970 9 In stock true just one stop codon in the end true Jonas Demeulemeester annotation1970253 1 cds range1970253 1 1 303 annotation1970251 1 start range1970251 1 1 3 annotation1970252 1 stop range1970252 1 304 306 BBa_E0020 1 ecfp engineered cyan fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_E0030 1 eyfp enhanced yellow fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Modificaitons to Clontech EYFP by Reshma Shetty Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_K272991 1 BBa_K272991 Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance marker-ECFP 2009-10-20T11:00:00Z 2015-07-09T01:03:33Z Registry of Standard Biological Parts Rbs-ccdB toxin - EYFP-constitutive promoter-Rbs-Streptomycin resistance marker-ECFP true false _369_ 4206 5014 9 Not in stock false Standard Assembly false IIT_Madras 2009 component2057886 1 BBa_E0020 component2057882 1 BBa_B0034 component2057880 1 BBa_J23119 component2057874 1 BBa_B0010 component2057876 1 BBa_B0012 component2057883 1 BBa_K156011 component2057866 1 BBa_B0034 component2057872 1 BBa_B0034 component2057873 1 BBa_E0030 component2057887 1 BBa_B0010 component2057870 1 BBa_K145151 component2057889 1 BBa_B0012 component2057885 1 BBa_B0034 annotation2057886 1 BBa_E0020 range2057886 1 2095 2817 annotation2057876 1 BBa_B0012 range2057876 1 1170 1210 annotation2057872 1 BBa_B0034 range2057872 1 333 344 annotation2057887 1 BBa_B0010 range2057887 1 2826 2905 annotation2057889 1 BBa_B0012 range2057889 1 2914 2954 annotation2057883 1 BBa_K156011 range2057883 1 1280 2068 annotation2057874 1 BBa_B0010 range2057874 1 1082 1161 annotation2057880 1 BBa_J23119 range2057880 1 1219 1253 annotation2057885 1 BBa_B0034 range2057885 1 2077 2088 annotation2057866 1 BBa_B0034 range2057866 1 1 12 annotation2057873 1 BBa_E0030 range2057873 1 351 1073 annotation2057882 1 BBa_B0034 range2057882 1 1262 1273 annotation2057870 1 BBa_K145151 range2057870 1 19 324 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K156011 1 BBa_K156011 aadA (streptomycin 3'-adenyltransferase) 2008-10-28T12:00:00Z 2015-05-08T01:10:54Z aadA was isolated from a plasmid in ''E. coli''. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438). This part codes for an aminoglycoside resistance protein that endows ''E. coli'' with resistance to the antibiotic streptomycin. This part can be used as a selection marker. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. true George McArthur IV BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_E0020_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K145151_sequence 1 atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_K272991_sequence 1 aaagaggagaaatactagatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcctggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtgatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0030_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_K156011_sequence 1 atgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcctggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z