BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K272992 1 BBa_K272992 Rbs-Streptomycin resistance marker-ECFP 2009-10-20T11:00:00Z 2015-05-08T01:11:44Z Registry of Standard Biological Parts Rbs-Streptomycin resistance marker-ECFP false false _369_ 0 5014 9 Not in stock false Standard Assembly false IIT_Madras 2009 component2057860 1 BBa_B0034 component2057861 1 BBa_K156011 annotation2057860 1 BBa_B0034 range2057860 1 1 12 annotation2057861 1 BBa_K156011 range2057861 1 19 807 BBa_K156011 1 BBa_K156011 aadA (streptomycin 3'-adenyltransferase) 2008-10-28T12:00:00Z 2015-05-08T01:10:54Z aadA was isolated from a plasmid in ''E. coli''. The original sequence was obtained from GenBank (VERSION NC_002525.1, GI:10955438). This part codes for an aminoglycoside resistance protein that endows ''E. coli'' with resistance to the antibiotic streptomycin. This part can be used as a selection marker. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. true George McArthur IV BBa_B0034_sequence 1 aaagaggagaaa BBa_K272992_sequence 1 aaagaggagaaatactagatgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcctggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtga BBa_K156011_sequence 1 atgcgcgaagcggttattgctgaagtttctactcaactgtctgaagttgtgggtgtaatcgaacgccacctggagccaaccctgctggctgtccacctgtacggctctgctgtggacggtggtctgaaaccacattccgacattgacctgctggtaactgtgaccgtgcgtctggacgaaactacccgtcgcgcgctgattaacgacctgctggaaacttctgcttctccgggtgaatccgaaatcctgcgtgcggtagaggttacgattgtggttcatgacgatattatcccgtggcgttaccctgctaagcgtgaactgcaatttggtgagtggcagcgcaacgacattctggcaggtatctttgaaccggcgaccatcgacatcgacctggctatcctgctgactaaagcgcgtgagcacagcgtagcgctggtgggccctgctgccgaggaactgttcgacccggttccggaacaagatctgttcgaagctctgaacgaaactctgaccctgtggaactctccgccggactgggcaggtgatgaacgcaacgtagttctgactctgtcccgcatttggtattccgctgtgaccggcaaaattgcccctaaagacgtcgctgcggattgggctatggaacgtctgcctgctcaatatcagccggtgatcctggaagcacgtcaggcgtacctgggccaggaggaccgcctggcaagccgtgctgaccagctggaagagttcgtacactacgttaagggcgagatcactaaggtagtgggtaagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z