LOCUS       BBa_K272993             1210 bp    DNA     linear       13-Jun-2024
DEFINITION  Rbs-ccdB toxin - EYFP
ACCESSION   BBa_K272993
VERSION     BBa_K272993.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=start
     misc_feature    322..324
                     /label=stop
     CDS             19..321
                     /label=cds
     CDS             19..324
                     /label=BBa_K145151
     misc_feature    337..340
                     /label=conserved
     RBS             333..344
                     /label=BBa_B0034
     CDS             351..1073
                     /label=BBa_E0030
     stem_loop       1093..1136
                     /label=stem_loop
     misc_feature    1082..1161
                     /label=BBa_B0010
     terminator      1082..1161
                     /label=BBa_B0010
     stem_loop       1177..1196
                     /label=T7 TE
     misc_feature    1203..1203
                     /label=stop
     polyA_site      1197..1210
                     /label=polya
     misc_feature    1170..1210
                     /label=BBa_B0012
     terminator      1170..1210
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gcagtttaag gtttacacct ataaaagaga gagccgttat
       61 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
      121 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
      181 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
      241 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
      301 aacctgatgt tctggggaat ataatactag agaaagagga gaaatactag atggtgagca
      361 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      421 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      481 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      541 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact
      601 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      661 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      721 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      781 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      841 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      901 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
      961 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1021 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga
     1081 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1141 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1201 tgcgtttata
//