LOCUS       BBa_K272997              926 bp    DNA     linear       20-Sep-2024
DEFINITION  Rbs-Spectinomycin resistance marker-terminator
ACCESSION   BBa_K272997
VERSION     BBa_K272997.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..783
                     /label=Aad9 Spectinomycin Acetyltransferase
     misc_feature    784..786
                     /label=stop
     misc_feature    787..789
                     /label=stop
     misc_feature    19..21
                     /label=start
     CDS             19..789
                     /label=BBa_K143031
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaggaggata tatttgaata catacgaaca aattaataaa
       61 gtgaaaaaaa tacttcggaa acatttaaaa aataacctta ttggtactta catgtttgga
      121 tcaggagttg agagtggact aaaaccaaat agtgatcttg actttttagt cgtcgtatct
      181 gaaccattga cagatcaaag taaagaaata cttatacaaa aaattagacc tatttcaaaa
      241 aaaataggag ataaaagcaa cttacgatat attgaattaa caattattat tcagcaagaa
      301 atggtaccgt ggaatcatcc tcccaaacaa gaatttattt atggagaatg gttacaagag
      361 ctttatgaac aaggatacat tcctcagaag gaattaaatt cagatttaac cataatgctt
      421 taccaagcaa aacgaaaaaa taaaagaata tacggaaatt atgacttaga ggaattacta
      481 cctgatattc cattttctga tgtgagaaga gccattatgg attcgtcaga ggaattaata
      541 gataattatc aggatgatga aaccaactct atattaactt tatgccgtat gattttaact
      601 atggacacgg gtaaaatcat accaaaagat attgcgggaa atgcagtggc tgaatcttct
      661 ccattagaac atagggagag aattttgtta gcagttcgta gttatcttgg agagaatatt
      721 gaatggacta atgaaaatgt aaatttaact ataaactatt taaataacag attaaaaaaa
      781 ttataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttata
//