BBa_K273006 1 kivd &#945;-ketoisovalerate decarboxylase (kivd) from <i>Lactococcus lactis</i> | codon optimized 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z Designed by the Uppsala iGEM Team 2009. Synthesized by Bioneer (www.bioneer.com) &#945;-ketoisovalerate decarboxylase (kivd) originally sequence from <i>Lactococcus lactis</i>. Codon optimized for use in <i>Synechocystis</i> and <i>E. coli</i>. false false _373_ 0 5250 9 It's complicated true The sequence was codon optimized using this [http://2009.igem.org/wiki/images/d/df/Codon_usage.txt codon-usage table] false Karl Brune annotation2047066 1 startcodon range2047066 1 1 3 annotation2047067 1 cds kivd_opt range2047067 1 1 1644 annotation2047068 1 stopcodon range2047068 1 1645 1650 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K273008 1 BBa_K273008 RBS - &#945;-ketoisovalerate decarboxylase (kivd) from Lactococcus lactis | codon optimized 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z Assembled by the Uppsala iGEM Team 2009 Intermediate construct for butanol production of RBS (B0034) and codon-omptimized &#945;-ketoisovalerate decarboxylase (kivd) from <i>Lactococcus lactis</i>. false false _373_ 0 5250 9 Not in stock false - false Karl Brune component2047105 1 BBa_B0034 component2047109 1 BBa_K273006 annotation2047105 1 BBa_B0034 range2047105 1 1 12 annotation2047109 1 BBa_K273006 range2047109 1 19 1668 BBa_K273006_sequence 1 atgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K273008_sequence 1 aaagaggagaaatactagatgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z