LOCUS       BBa_K273011             2944 bp    DNA     linear       26-Jun-2024
DEFINITION  PLlac - RBS - kivd - RBS - adh2(Y) - Ter
ACCESSION   BBa_K273011
VERSION     BBa_K273011.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=startcodon
     CDS             82..1725
                     /label=cds kivd_opt
     misc_feature    1726..1731
                     /label=stopcodon
     CDS             82..1731
                     /label=BBa_K273006
     misc_feature    1744..1747
                     /label=conserved
     RBS             1740..1751
                     /label=BBa_B0034
     misc_feature    1758..1760
                     /label=startcodon
     CDS             1758..2801
                     /label=cds
     misc_feature    2802..2807
                     /label=stopcodon
     CDS             1758..2807
                     /label=BBa_K273005
     stem_loop       2827..2870
                     /label=stem_loop
     misc_feature    2816..2895
                     /label=BBa_B0010
     terminator      2816..2895
                     /label=BBa_B0010
     stem_loop       2911..2930
                     /label=T7 TE
     misc_feature    2937..2937
                     /label=stop
     polyA_site      2931..2944
                     /label=polya
     misc_feature    2904..2944
                     /label=BBa_B0012
     terminator      2904..2944
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtatact gttggtgatt atctgttgga ccgtctgcat
      121 gagctgggca ttgaagaaat ctttggtgtg ccgggtgatt acaatctgca atttctggat
      181 cagattatca gtcataaaga tatgaaatgg gtgggcaacg cgaacgaact gaacgcgagc
      241 tacatggccg atggttatgc gcgcactaag aaagccgcgg ccttcctgac cacctttggt
      301 gtgggtgaac tgagcgccgt gaatggcctg gcgggtagct acgccgaaaa tctgcccgtg
      361 gtggaaatcg tgggcagccc gactagcaag gttcagaacg aaggcaagtt cgttcatcat
      421 accctggccg acggcgattt caaacacttt atgaaaatgc acgaacccgt tactgcggcg
      481 cgtactctgt tgaccgcgga gaacgccacc gtggaaatcg atcgtgttct gagcgccctg
      541 ttgaaagagc gcaagcccgt gtatatcaat ctgccggttg atgttgccgc ggccaaagcg
      601 gaaaaaccga gtctgcccct gaaaaaggaa aatagtacca gtaataccag cgatcaggag
      661 atcctgaaca agatccagga aagcctgaaa aatgccaaga aaccgatcgt gattactggc
      721 catgagatta ttagtttcgg tctggagaaa accgtgaccc aattcattag caaaaccaaa
      781 ctgcccatta ccaccctgaa cttcggtaaa agcagcgttg atgaggcgct gccgagcttt
      841 ctgggcattt acaatggtac cctgagcgaa cccaacctga aagagtttgt tgaaagtgcg
      901 gatttcattc tgatgctggg tgttaaactg accgatagta gtaccggcgc cttcactcat
      961 catctgaatg aaaataagat gatcagtctg aacattgatg aaggcaagat tttcaacgaa
     1021 cgtatccaaa actttgattt tgagagtctg atcagtagtc tgttggacct gagcgagatt
     1081 gaatacaagg gcaagtacat tgacaagaag caagaagatt tcgttccgag taatgccctg
     1141 ttgagtcaag atcgcctgtg gcaagcggtg gaaaatctga cccaaagcaa cgaaaccatt
     1201 gtggccgaac agggcaccag ctttttcggt gccagtagta tttttctgaa aagtaagagc
     1261 cacttcatcg gtcaaccgct gtggggcagc attggctata cttttccggc cgccctgggc
     1321 agccaaatcg ccgataaaga aagccgccat ctgttgttta ttggtgatgg cagcctgcaa
     1381 ctgaccgttc aagagctggg cctggccatt cgtgagaaga ttaatccgat ttgtttcatt
     1441 atcaacaacg atggctatac tgttgaacgt gagattcatg gtccgaacca gagttataat
     1501 gacattccga tgtggaacta cagcaagctg ccggaaagct tcggcgccac cgaagatcgc
     1561 gtggtgagta agattgtgcg tactgagaac gagttcgtta gtgtgatgaa agaggcgcaa
     1621 gccgatccga atcgtatgta ttggattgag ctgattctgg cgaaagaggg tgcgccgaag
     1681 gttctgaaaa agatgggtaa actgtttgcg gagcagaaca aaagttaata atactagaga
     1741 aagaggagaa atactagatg tctattccag aaactcaaaa agccattatc ttctacgaat
     1801 ccaacggcaa gttggagcat aaggatatcc cagttccaaa gccaaagccc aacgaattgt
     1861 taatcaacgt caagtactct ggtgtctgcc acaccgattt gcacgcttgg catggtgact
     1921 ggccattgcc aactaagtta ccattagttg gtggtcacga aggtgccggt gtcgttgtcg
     1981 gcatgggtga aaacgttaag ggctggaaga tcggtgacta cgccggtatc aaatggttga
     2041 acggttcttg tatggcctgt gaatactgtg aattgggtaa cgaatccaac tgtcctcacg
     2101 ctgacttgtc tggttacacc cacgacggtt ctttccaaga atacgctacc gctgacgctg
     2161 ttcaagccgc tcacattcct caaggtactg acttggctga agtcgcgcca atcttgtgtg
     2221 ctggtatcac cgtatacaag gctttgaagt ctgccaactt gagagcaggc cactgggcgg
     2281 ccatttctgg tgctgctggt ggtctaggtt ctttggctgt tcaatatgct aaggcgatgg
     2341 gttacagagt cttaggtatt gatggtggtc caggaaagga agaattgttt acctcgctcg
     2401 gtggtgaagt attcatcgac ttcaccaaag agaaggacat tgttagcgca gtcgttaagg
     2461 ctaccaacgg cggtgcccac ggtatcatca atgtttccgt ttccgaagcc gctatcgaag
     2521 cttctaccag atactgtagg gcgaacggta ctgttgtctt ggttggtttg ccagccggtg
     2581 caaagtgctc ctctgatgtc ttcaaccacg ttgtcaagtc tatctccatt gtcggctctt
     2641 acgtggggaa cagagctgat accagagaag ccttagattt ctttgccaga ggtctagtca
     2701 agtctccaat aaaggtagtt ggcttatcca gtttaccaga aatttacgaa aagatggaga
     2761 agggccaaat tgctggtaga tacgttgttg acacttctaa ataataatac tagagccagg
     2821 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2881 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2941 tata
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