BBa_K273011 1 BBa_K273011 PLlac - RBS - kivd - RBS - adh2(Y) - Ter 2009-10-16T11:00:00Z 2015-05-08T01:11:44Z Assembled by the Uppsala iGEM Team 2009 Construct for testing and characterization purposes in <i>E. coli</i>. false false _373_ 0 5250 9 It's complicated false - false Karl Brune component2048561 1 BBa_B0034 component2048565 1 BBa_K273005 component2048566 1 BBa_B0010 component2048568 1 BBa_B0012 component2048549 1 BBa_R0011 component2048559 1 BBa_K273006 component2048555 1 BBa_B0034 annotation2048568 1 BBa_B0012 range2048568 1 2904 2944 annotation2048555 1 BBa_B0034 range2048555 1 64 75 annotation2048561 1 BBa_B0034 range2048561 1 1740 1751 annotation2048549 1 BBa_R0011 range2048549 1 1 54 annotation2048559 1 BBa_K273006 range2048559 1 82 1731 annotation2048566 1 BBa_B0010 range2048566 1 2816 2895 annotation2048565 1 BBa_K273005 range2048565 1 1758 2807 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 BBa_K273005 1 adh2 Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from <i>S. cerevisiae</i> 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z <i>S. cerevisiae</i> Alcohol Dehydrogenase 2 from <i>S. cerevisiae</i> false false _373_ 0 5250 9 It's complicated true - false Karl Brune annotation2046975 1 startcodon range2046975 1 1 3 annotation2046976 1 cds range2046976 1 1 1044 annotation2046992 1 stopcodon range2046992 1 1045 1050 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2001 1 lac O1 range2001 1 26 42 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K273006 1 kivd &#945;-ketoisovalerate decarboxylase (kivd) from <i>Lactococcus lactis</i> | codon optimized 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z Designed by the Uppsala iGEM Team 2009. Synthesized by Bioneer (www.bioneer.com) &#945;-ketoisovalerate decarboxylase (kivd) originally sequence from <i>Lactococcus lactis</i>. Codon optimized for use in <i>Synechocystis</i> and <i>E. coli</i>. false false _373_ 0 5250 9 It's complicated true The sequence was codon optimized using this [http://2009.igem.org/wiki/images/d/df/Codon_usage.txt codon-usage table] false Karl Brune annotation2047066 1 startcodon range2047066 1 1 3 annotation2047068 1 stopcodon range2047068 1 1645 1650 annotation2047067 1 cds kivd_opt range2047067 1 1 1644 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K273006_sequence 1 atgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K273005_sequence 1 atgtctattccagaaactcaaaaagccattatcttctacgaatccaacggcaagttggagcataaggatatcccagttccaaagccaaagcccaacgaattgttaatcaacgtcaagtactctggtgtctgccacaccgatttgcacgcttggcatggtgactggccattgccaactaagttaccattagttggtggtcacgaaggtgccggtgtcgttgtcggcatgggtgaaaacgttaagggctggaagatcggtgactacgccggtatcaaatggttgaacggttcttgtatggcctgtgaatactgtgaattgggtaacgaatccaactgtcctcacgctgacttgtctggttacacccacgacggttctttccaagaatacgctaccgctgacgctgttcaagccgctcacattcctcaaggtactgacttggctgaagtcgcgccaatcttgtgtgctggtatcaccgtatacaaggctttgaagtctgccaacttgagagcaggccactgggcggccatttctggtgctgctggtggtctaggttctttggctgttcaatatgctaaggcgatgggttacagagtcttaggtattgatggtggtccaggaaaggaagaattgtttacctcgctcggtggtgaagtattcatcgacttcaccaaagagaaggacattgttagcgcagtcgttaaggctaccaacggcggtgcccacggtatcatcaatgtttccgtttccgaagccgctatcgaagcttctaccagatactgtagggcgaacggtactgttgtcttggttggtttgccagccggtgcaaagtgctcctctgatgtcttcaaccacgttgtcaagtctatctccattgtcggctcttacgtggggaacagagctgataccagagaagccttagatttctttgccagaggtctagtcaagtctccaataaaggtagttggcttatccagtttaccagaaatttacgaaaagatggagaagggccaaattgctggtagatacgttgttgacacttctaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_K273011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataatactagagaaagaggagaaatactagatgtctattccagaaactcaaaaagccattatcttctacgaatccaacggcaagttggagcataaggatatcccagttccaaagccaaagcccaacgaattgttaatcaacgtcaagtactctggtgtctgccacaccgatttgcacgcttggcatggtgactggccattgccaactaagttaccattagttggtggtcacgaaggtgccggtgtcgttgtcggcatgggtgaaaacgttaagggctggaagatcggtgactacgccggtatcaaatggttgaacggttcttgtatggcctgtgaatactgtgaattgggtaacgaatccaactgtcctcacgctgacttgtctggttacacccacgacggttctttccaagaatacgctaccgctgacgctgttcaagccgctcacattcctcaaggtactgacttggctgaagtcgcgccaatcttgtgtgctggtatcaccgtatacaaggctttgaagtctgccaacttgagagcaggccactgggcggccatttctggtgctgctggtggtctaggttctttggctgttcaatatgctaaggcgatgggttacagagtcttaggtattgatggtggtccaggaaaggaagaattgtttacctcgctcggtggtgaagtattcatcgacttcaccaaagagaaggacattgttagcgcagtcgttaaggctaccaacggcggtgcccacggtatcatcaatgtttccgtttccgaagccgctatcgaagcttctaccagatactgtagggcgaacggtactgttgtcttggttggtttgccagccggtgcaaagtgctcctctgatgtcttcaaccacgttgtcaagtctatctccattgtcggctcttacgtggggaacagagctgataccagagaagccttagatttctttgccagaggtctagtcaagtctccaataaaggtagttggcttatccagtttaccagaaatttacgaaaagatggagaagggccaaattgctggtagatacgttgttgacacttctaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z