LOCUS       BBa_K273012             3318 bp    DNA     linear       18-Feb-2025
DEFINITION  pPetE - RBS - kivd - RBS - adh2(Y) - Ter
ACCESSION   BBa_K273012
VERSION     BBa_K273012.1  
FEATURES             Location/Qualifiers
     promoter        1..429
                     /label=BBa_K273019
     misc_feature    442..445
                     /label=conserved
     RBS             438..449
                     /label=BBa_B0034
     misc_feature    456..458
                     /label=startcodon
     CDS             456..2099
                     /label=cds kivd_opt
     misc_feature    2100..2105
                     /label=stopcodon
     CDS             456..2105
                     /label=BBa_K273006
     misc_feature    2118..2121
                     /label=conserved
     RBS             2114..2125
                     /label=BBa_B0034
     misc_feature    2132..2134
                     /label=startcodon
     CDS             2132..3175
                     /label=cds
     misc_feature    3176..3181
                     /label=stopcodon
     CDS             2132..3181
                     /label=BBa_K273005
     stem_loop       3201..3244
                     /label=stem_loop
     misc_feature    3190..3269
                     /label=BBa_B0010
     terminator      3190..3269
                     /label=BBa_B0010
     stem_loop       3285..3304
                     /label=T7 TE
     misc_feature    3311..3311
                     /label=stop
     polyA_site      3305..3318
                     /label=polya
     misc_feature    3278..3318
                     /label=BBa_B0012
     terminator      3278..3318
                     /label=BBa_B0012
ORIGIN
        1 tcatagcggt tgcccaatct aactcaggga gcgacttcag cccacaaaaa acaccactgg
       61 gcctactggg ctattcccat tatcatctac attgaaggga tagcaagcta atttttatga
      121 cggcgatcgc caaaaacaaa gaaaattcag caattaccgt gggtagcaaa aaatccccat
      181 ctaaagttca gtaaatatag ctagaacaac caagcatttt cggcaaagta ctattcagat
      241 agaacgagaa atgagcttgt tctatccgcc cggggctgag gctgtataat ctacgacggg
      301 ctgtcaaaca ttgtgatacc atgggcagaa gaaaggaaaa acgtccctga tcgccttttt
      361 gggcacggag tagggcgtta ccccggcccg ttcaaccaca agtccctata gatacaatcg
      421 ccaagaagtt actagagaaa gaggagaaat actagatgta tactgttggt gattatctgt
      481 tggaccgtct gcatgagctg ggcattgaag aaatctttgg tgtgccgggt gattacaatc
      541 tgcaatttct ggatcagatt atcagtcata aagatatgaa atgggtgggc aacgcgaacg
      601 aactgaacgc gagctacatg gccgatggtt atgcgcgcac taagaaagcc gcggccttcc
      661 tgaccacctt tggtgtgggt gaactgagcg ccgtgaatgg cctggcgggt agctacgccg
      721 aaaatctgcc cgtggtggaa atcgtgggca gcccgactag caaggttcag aacgaaggca
      781 agttcgttca tcataccctg gccgacggcg atttcaaaca ctttatgaaa atgcacgaac
      841 ccgttactgc ggcgcgtact ctgttgaccg cggagaacgc caccgtggaa atcgatcgtg
      901 ttctgagcgc cctgttgaaa gagcgcaagc ccgtgtatat caatctgccg gttgatgttg
      961 ccgcggccaa agcggaaaaa ccgagtctgc ccctgaaaaa ggaaaatagt accagtaata
     1021 ccagcgatca ggagatcctg aacaagatcc aggaaagcct gaaaaatgcc aagaaaccga
     1081 tcgtgattac tggccatgag attattagtt tcggtctgga gaaaaccgtg acccaattca
     1141 ttagcaaaac caaactgccc attaccaccc tgaacttcgg taaaagcagc gttgatgagg
     1201 cgctgccgag ctttctgggc atttacaatg gtaccctgag cgaacccaac ctgaaagagt
     1261 ttgttgaaag tgcggatttc attctgatgc tgggtgttaa actgaccgat agtagtaccg
     1321 gcgccttcac tcatcatctg aatgaaaata agatgatcag tctgaacatt gatgaaggca
     1381 agattttcaa cgaacgtatc caaaactttg attttgagag tctgatcagt agtctgttgg
     1441 acctgagcga gattgaatac aagggcaagt acattgacaa gaagcaagaa gatttcgttc
     1501 cgagtaatgc cctgttgagt caagatcgcc tgtggcaagc ggtggaaaat ctgacccaaa
     1561 gcaacgaaac cattgtggcc gaacagggca ccagcttttt cggtgccagt agtatttttc
     1621 tgaaaagtaa gagccacttc atcggtcaac cgctgtgggg cagcattggc tatacttttc
     1681 cggccgccct gggcagccaa atcgccgata aagaaagccg ccatctgttg tttattggtg
     1741 atggcagcct gcaactgacc gttcaagagc tgggcctggc cattcgtgag aagattaatc
     1801 cgatttgttt cattatcaac aacgatggct atactgttga acgtgagatt catggtccga
     1861 accagagtta taatgacatt ccgatgtgga actacagcaa gctgccggaa agcttcggcg
     1921 ccaccgaaga tcgcgtggtg agtaagattg tgcgtactga gaacgagttc gttagtgtga
     1981 tgaaagaggc gcaagccgat ccgaatcgta tgtattggat tgagctgatt ctggcgaaag
     2041 agggtgcgcc gaaggttctg aaaaagatgg gtaaactgtt tgcggagcag aacaaaagtt
     2101 aataatacta gagaaagagg agaaatacta gatgtctatt ccagaaactc aaaaagccat
     2161 tatcttctac gaatccaacg gcaagttgga gcataaggat atcccagttc caaagccaaa
     2221 gcccaacgaa ttgttaatca acgtcaagta ctctggtgtc tgccacaccg atttgcacgc
     2281 ttggcatggt gactggccat tgccaactaa gttaccatta gttggtggtc acgaaggtgc
     2341 cggtgtcgtt gtcggcatgg gtgaaaacgt taagggctgg aagatcggtg actacgccgg
     2401 tatcaaatgg ttgaacggtt cttgtatggc ctgtgaatac tgtgaattgg gtaacgaatc
     2461 caactgtcct cacgctgact tgtctggtta cacccacgac ggttctttcc aagaatacgc
     2521 taccgctgac gctgttcaag ccgctcacat tcctcaaggt actgacttgg ctgaagtcgc
     2581 gccaatcttg tgtgctggta tcaccgtata caaggctttg aagtctgcca acttgagagc
     2641 aggccactgg gcggccattt ctggtgctgc tggtggtcta ggttctttgg ctgttcaata
     2701 tgctaaggcg atgggttaca gagtcttagg tattgatggt ggtccaggaa aggaagaatt
     2761 gtttacctcg ctcggtggtg aagtattcat cgacttcacc aaagagaagg acattgttag
     2821 cgcagtcgtt aaggctacca acggcggtgc ccacggtatc atcaatgttt ccgtttccga
     2881 agccgctatc gaagcttcta ccagatactg tagggcgaac ggtactgttg tcttggttgg
     2941 tttgccagcc ggtgcaaagt gctcctctga tgtcttcaac cacgttgtca agtctatctc
     3001 cattgtcggc tcttacgtgg ggaacagagc tgataccaga gaagccttag atttctttgc
     3061 cagaggtcta gtcaagtctc caataaaggt agttggctta tccagtttac cagaaattta
     3121 cgaaaagatg gagaagggcc aaattgctgg tagatacgtt gttgacactt ctaaataata
     3181 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3241 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     3301 ggcctttctg cgtttata
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