BBa_K273006 1 kivd &#945;-ketoisovalerate decarboxylase (kivd) from <i>Lactococcus lactis</i> | codon optimized 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z Designed by the Uppsala iGEM Team 2009. Synthesized by Bioneer (www.bioneer.com) &#945;-ketoisovalerate decarboxylase (kivd) originally sequence from <i>Lactococcus lactis</i>. Codon optimized for use in <i>Synechocystis</i> and <i>E. coli</i>. false false _373_ 0 5250 9 It's complicated true The sequence was codon optimized using this [http://2009.igem.org/wiki/images/d/df/Codon_usage.txt codon-usage table] false Karl Brune annotation2047068 1 stopcodon range2047068 1 1645 1650 annotation2047066 1 startcodon range2047066 1 1 3 annotation2047067 1 cds kivd_opt range2047067 1 1 1644 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_K273005 1 adh2 Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from <i>S. cerevisiae</i> 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z <i>S. cerevisiae</i> Alcohol Dehydrogenase 2 from <i>S. cerevisiae</i> false false _373_ 0 5250 9 It's complicated true - false Karl Brune annotation2046992 1 stopcodon range2046992 1 1045 1050 annotation2046976 1 cds range2046976 1 1 1044 annotation2046975 1 startcodon range2046975 1 1 3 BBa_K273012 1 BBa_K273012 pPetE - RBS - kivd - RBS - adh2(Y) - Ter 2009-10-17T11:00:00Z 2015-05-08T01:11:44Z - - false false _373_ 0 5250 9 It's complicated false - false Karl Brune component2050236 1 BBa_B0034 component2050230 1 BBa_B0034 component2050243 1 BBa_B0012 component2050234 1 BBa_K273006 component2050228 1 BBa_K273019 component2050240 1 BBa_K273005 component2050241 1 BBa_B0010 annotation2050240 1 BBa_K273005 range2050240 1 2132 3181 annotation2050234 1 BBa_K273006 range2050234 1 456 2105 annotation2050241 1 BBa_B0010 range2050241 1 3190 3269 annotation2050236 1 BBa_B0034 range2050236 1 2114 2125 annotation2050243 1 BBa_B0012 range2050243 1 3278 3318 annotation2050228 1 BBa_K273019 range2050228 1 1 429 annotation2050230 1 BBa_B0034 range2050230 1 438 449 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K273019 1 PpetE PpetE 2009-10-17T11:00:00Z 2015-05-08T01:11:44Z - pPetE copper inducible promoter false false _373_ 0 5250 9 It's complicated true - false Karl Brune BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K273006_sequence 1 atgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K273019_sequence 1 tcatagcggttgcccaatctaactcagggagcgacttcagcccacaaaaaacaccactgggcctactgggctattcccattatcatctacattgaagggatagcaagctaatttttatgacggcgatcgccaaaaacaaagaaaattcagcaattaccgtgggtagcaaaaaatccccatctaaagttcagtaaatatagctagaacaaccaagcattttcggcaaagtactattcagatagaacgagaaatgagcttgttctatccgcccggggctgaggctgtataatctacgacgggctgtcaaacattgtgataccatgggcagaagaaaggaaaaacgtccctgatcgcctttttgggcacggagtagggcgttaccccggcccgttcaaccacaagtccctatagatacaatcgccaagaagt BBa_K273005_sequence 1 atgtctattccagaaactcaaaaagccattatcttctacgaatccaacggcaagttggagcataaggatatcccagttccaaagccaaagcccaacgaattgttaatcaacgtcaagtactctggtgtctgccacaccgatttgcacgcttggcatggtgactggccattgccaactaagttaccattagttggtggtcacgaaggtgccggtgtcgttgtcggcatgggtgaaaacgttaagggctggaagatcggtgactacgccggtatcaaatggttgaacggttcttgtatggcctgtgaatactgtgaattgggtaacgaatccaactgtcctcacgctgacttgtctggttacacccacgacggttctttccaagaatacgctaccgctgacgctgttcaagccgctcacattcctcaaggtactgacttggctgaagtcgcgccaatcttgtgtgctggtatcaccgtatacaaggctttgaagtctgccaacttgagagcaggccactgggcggccatttctggtgctgctggtggtctaggttctttggctgttcaatatgctaaggcgatgggttacagagtcttaggtattgatggtggtccaggaaaggaagaattgtttacctcgctcggtggtgaagtattcatcgacttcaccaaagagaaggacattgttagcgcagtcgttaaggctaccaacggcggtgcccacggtatcatcaatgtttccgtttccgaagccgctatcgaagcttctaccagatactgtagggcgaacggtactgttgtcttggttggtttgccagccggtgcaaagtgctcctctgatgtcttcaaccacgttgtcaagtctatctccattgtcggctcttacgtggggaacagagctgataccagagaagccttagatttctttgccagaggtctagtcaagtctccaataaaggtagttggcttatccagtttaccagaaatttacgaaaagatggagaagggccaaattgctggtagatacgttgttgacacttctaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K273012_sequence 1 tcatagcggttgcccaatctaactcagggagcgacttcagcccacaaaaaacaccactgggcctactgggctattcccattatcatctacattgaagggatagcaagctaatttttatgacggcgatcgccaaaaacaaagaaaattcagcaattaccgtgggtagcaaaaaatccccatctaaagttcagtaaatatagctagaacaaccaagcattttcggcaaagtactattcagatagaacgagaaatgagcttgttctatccgcccggggctgaggctgtataatctacgacgggctgtcaaacattgtgataccatgggcagaagaaaggaaaaacgtccctgatcgcctttttgggcacggagtagggcgttaccccggcccgttcaaccacaagtccctatagatacaatcgccaagaagttactagagaaagaggagaaatactagatgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataatactagagaaagaggagaaatactagatgtctattccagaaactcaaaaagccattatcttctacgaatccaacggcaagttggagcataaggatatcccagttccaaagccaaagcccaacgaattgttaatcaacgtcaagtactctggtgtctgccacaccgatttgcacgcttggcatggtgactggccattgccaactaagttaccattagttggtggtcacgaaggtgccggtgtcgttgtcggcatgggtgaaaacgttaagggctggaagatcggtgactacgccggtatcaaatggttgaacggttcttgtatggcctgtgaatactgtgaattgggtaacgaatccaactgtcctcacgctgacttgtctggttacacccacgacggttctttccaagaatacgctaccgctgacgctgttcaagccgctcacattcctcaaggtactgacttggctgaagtcgcgccaatcttgtgtgctggtatcaccgtatacaaggctttgaagtctgccaacttgagagcaggccactgggcggccatttctggtgctgctggtggtctaggttctttggctgttcaatatgctaaggcgatgggttacagagtcttaggtattgatggtggtccaggaaaggaagaattgtttacctcgctcggtggtgaagtattcatcgacttcaccaaagagaaggacattgttagcgcagtcgttaaggctaccaacggcggtgcccacggtatcatcaatgtttccgtttccgaagccgctatcgaagcttctaccagatactgtagggcgaacggtactgttgtcttggttggtttgccagccggtgcaaagtgctcctctgatgtcttcaaccacgttgtcaagtctatctccattgtcggctcttacgtggggaacagagctgataccagagaagccttagatttctttgccagaggtctagtcaagtctccaataaaggtagttggcttatccagtttaccagaaatttacgaaaagatggagaagggccaaattgctggtagatacgttgttgacacttctaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z