LOCUS       BBa_K273013             3046 bp    DNA     linear       27-Jun-2024
DEFINITION  RBS - pdc - RBS - adh2(Z) - Ter
ACCESSION   BBa_K273013
VERSION     BBa_K273013.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=startcodon
     misc_feature    1723..1728
                     /label=stopcodon (double)
     CDS             19..1722
                     /label=coding sequence
     CDS             19..1728
                     /label=BBa_K273000
     misc_feature    1741..1744
                     /label=conserved
     RBS             1737..1748
                     /label=BBa_B0034
     misc_feature    1755..1757
                     /label=startcodon
     misc_feature    2904..2909
                     /label=stopcodon (double)
     CDS             1755..2903
                     /label=cds adh2
     CDS             1755..2909
                     /label=BBa_K273001
     stem_loop       2929..2972
                     /label=stem_loop
     misc_feature    2918..2997
                     /label=BBa_B0010
     terminator      2918..2997
                     /label=BBa_B0010
     stem_loop       3013..3032
                     /label=T7 TE
     misc_feature    3039..3039
                     /label=stop
     polyA_site      3033..3046
                     /label=polya
     misc_feature    3006..3046
                     /label=BBa_B0012
     terminator      3006..3046
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagttatact gtcggtacct atttagcgga gcggcttgtc
       61 cagattggtc tcaagcatca cttcgcagtc gcgggcgact acaacctcgt ccttcttgac
      121 aacctgcttt tgaacaaaaa catggagcag gtttattgct gtaacgaact gaactgcggt
      181 ttcagtgcag aaggttatgc tcgtgccaaa ggcgcagcag cagccgtcgt tacctacagc
      241 gtcggtgcgc tttccgcatt tgatgctatc ggtggcgcct atgcagaaaa ccttccggtt
      301 atcctgatct ccggtgctcc gaacaacaat gatcacgctg ctggtcacgt gttgcatcac
      361 gctcttggca aaaccgacta tcactatcag ttggaaatgg ccaagaacat cacggccgcc
      421 gctgaagcga tttacacccc ggaagaagct ccggctaaaa tcgatcacgt gattaaaact
      481 gctcttcgtg agaagaagcc ggtttatctc gaaatcgctt gcaacattgc ttccatgccc
      541 tgcgccgctc ctggaccggc aagcgcattg ttcaatgacg aagccagcga cgaagcttct
      601 ttgaatgcag cggttgaaga aaccctgaaa ttcatcgcca accgcgacaa agttgccgtc
      661 ctcgtcggca gcaagctgcg cgcagctggt gctgaagaag ctgctgtcaa atttgctgat
      721 gctctcggtg gcgcagttgc taccatggct gctgcaaaaa gcttcttccc agaagaaaac
      781 ccgcattaca tcggcacctc atggggtgaa gtcagctatc cgggcgttga aaagacgatg
      841 aaagaagccg atgcggttat cgctctggct cctgtcttca acgactactc caccactggt
      901 tggacggata ttcctgatcc taagaaactg gttctcgctg aaccgcgttc tgtcgtcgtt
      961 aacggcattc gcttccccag cgtccatctg aaagactatc tgacccgttt ggctcagaaa
     1021 gtttccaaga aaaccggtgc attggacttc ttcaaatccc tcaatgcagg tgaactgaag
     1081 aaagccgctc cggctgatcc gagtgctccg ttggtcaacg cagaaatcgc ccgtcaggtc
     1141 gaagctcttc tgaccccgaa cacgacggtt attgctgaaa ccggtgactc ttggttcaat
     1201 gctcagcgca tgaagctccc gaacggtgct cgcgttgaat atgaaatgca gtggggtcac
     1261 attggttggt ccgttcctgc cgccttcggt tatgccgtcg gtgctccgga acgtcgcaac
     1321 atcctcatgg ttggtgatgg ttccttccag ctgacggctc aggaagtcgc tcagatggtt
     1381 cgcctgaaac tgccggttat catcttcttg atcaataact atggttacac catcgaagtt
     1441 atgatccatg atggtccgta caacaacatc aagaactggg attatgccgg tctgatggaa
     1501 gtgttcaacg gtaacggtgg ttatgacagc ggtgctggta aaggcctgaa ggctaaaacc
     1561 ggtggcgaac tggcagaagc tatcaaggtt gctctggcaa acaccgacgg cccaaccctg
     1621 atcgaatgct tcatcggtcg tgaagactgc actgaagaat tggtcaaatg gggtaagcgc
     1681 gttgctgccg ccaacagccg taagcctgtt aacaagctcc tctaataata ctagagaaag
     1741 aggagaaata ctagatggct tcttcaactt tttatattcc tttcgtcaac gaaatgggcg
     1801 aaggttcgct tgaaaaagca atcaaggatc ttaacggcag cggctttaaa aatgcgctga
     1861 tcgtttctga tgctttcatg aacaaatccg gtgttgtgaa gcaggttgct gacctgttga
     1921 aagcacaggg tattaattct gctgtttatg atggcgttat gccgaacccg actgttaccg
     1981 cagttctgga aggccttaag atcctgaagg ataacaattc agacttcgtc atctccctcg
     2041 gtggtggttc tccccatgac tgcgccaaag ccatcgctct ggtcgcaacc aatggtggtg
     2101 aagtcaaaga ctacgaaggt atcgacaaat ctaagaaacc tgccctgcct ttgatgtcaa
     2161 tcaacacgac ggctggtacg gcttctgaaa tgacgcgttt ctgcatcatc actgatgaag
     2221 tccgtcacgt taagatggcc attgttgacc gtcacgttac cccgatggtt tccgtcaacg
     2281 atcctctgtt gatggttggt atgccaaaag gcctgaccgc cgccaccggt atggatgctc
     2341 tgacccacgc atttgaagct tattcttcaa cggcagctac tccgatcacc gatgcttgcg
     2401 ctttgaaagc agcttccatg atcgctaaga atctgaagac cgcttgcgac aacggtaagg
     2461 atatgccggc tcgtgaagct atggcttatg cccaattcct cgctggtatg gccttcaaca
     2521 acgcttcgct tggttatgtc catgctatgg ctcaccagtt gggcggttac tacaacctgc
     2581 cgcatggtgt ctgcaacgct gttctgcttc cgcatgttct ggcttataac gcctctgtcg
     2641 ttgctggtcg tctgaaagac gttggtgttg ctatgggtct cgatatcgcc aatctcggtg
     2701 ataaagaagg cgcagaagcc accattcagg ctgttcgcga tctggctgct tccattggta
     2761 ttccagcaaa cctgaccgag ctgggtgcta agaaagaaga tgtgccgctt cttgctgacc
     2821 acgctctgaa agatgcttgt gctctgacca acccgcgtca gggtgatcag aaagaagttg
     2881 aagaactctt cctgagcgct ttctaataat actagagcca ggcatcaaat aaaacgaaag
     2941 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     3001 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//