LOCUS       BBa_K273015             3004 bp    DNA     linear       26-Nov-2024
DEFINITION  PLlac - RBS - pdc - RBS - adh2(Y) - Ter
ACCESSION   BBa_K273015
VERSION     BBa_K273015.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=startcodon
     misc_feature    1786..1791
                     /label=stopcodon (double)
     CDS             82..1785
                     /label=coding sequence
     CDS             82..1791
                     /label=BBa_K273000
     misc_feature    1804..1807
                     /label=conserved
     RBS             1800..1811
                     /label=BBa_B0034
     misc_feature    1818..1820
                     /label=startcodon
     CDS             1818..2861
                     /label=cds
     misc_feature    2862..2867
                     /label=stopcodon
     CDS             1818..2867
                     /label=BBa_K273005
     stem_loop       2887..2930
                     /label=stem_loop
     misc_feature    2876..2955
                     /label=BBa_B0010
     terminator      2876..2955
                     /label=BBa_B0010
     stem_loop       2971..2990
                     /label=T7 TE
     misc_feature    2997..2997
                     /label=stop
     polyA_site      2991..3004
                     /label=polya
     misc_feature    2964..3004
                     /label=BBa_B0012
     terminator      2964..3004
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagttat actgtcggta cctatttagc ggagcggctt
      121 gtccagattg gtctcaagca tcacttcgca gtcgcgggcg actacaacct cgtccttctt
      181 gacaacctgc ttttgaacaa aaacatggag caggtttatt gctgtaacga actgaactgc
      241 ggtttcagtg cagaaggtta tgctcgtgcc aaaggcgcag cagcagccgt cgttacctac
      301 agcgtcggtg cgctttccgc atttgatgct atcggtggcg cctatgcaga aaaccttccg
      361 gttatcctga tctccggtgc tccgaacaac aatgatcacg ctgctggtca cgtgttgcat
      421 cacgctcttg gcaaaaccga ctatcactat cagttggaaa tggccaagaa catcacggcc
      481 gccgctgaag cgatttacac cccggaagaa gctccggcta aaatcgatca cgtgattaaa
      541 actgctcttc gtgagaagaa gccggtttat ctcgaaatcg cttgcaacat tgcttccatg
      601 ccctgcgccg ctcctggacc ggcaagcgca ttgttcaatg acgaagccag cgacgaagct
      661 tctttgaatg cagcggttga agaaaccctg aaattcatcg ccaaccgcga caaagttgcc
      721 gtcctcgtcg gcagcaagct gcgcgcagct ggtgctgaag aagctgctgt caaatttgct
      781 gatgctctcg gtggcgcagt tgctaccatg gctgctgcaa aaagcttctt cccagaagaa
      841 aacccgcatt acatcggcac ctcatggggt gaagtcagct atccgggcgt tgaaaagacg
      901 atgaaagaag ccgatgcggt tatcgctctg gctcctgtct tcaacgacta ctccaccact
      961 ggttggacgg atattcctga tcctaagaaa ctggttctcg ctgaaccgcg ttctgtcgtc
     1021 gttaacggca ttcgcttccc cagcgtccat ctgaaagact atctgacccg tttggctcag
     1081 aaagtttcca agaaaaccgg tgcattggac ttcttcaaat ccctcaatgc aggtgaactg
     1141 aagaaagccg ctccggctga tccgagtgct ccgttggtca acgcagaaat cgcccgtcag
     1201 gtcgaagctc ttctgacccc gaacacgacg gttattgctg aaaccggtga ctcttggttc
     1261 aatgctcagc gcatgaagct cccgaacggt gctcgcgttg aatatgaaat gcagtggggt
     1321 cacattggtt ggtccgttcc tgccgccttc ggttatgccg tcggtgctcc ggaacgtcgc
     1381 aacatcctca tggttggtga tggttccttc cagctgacgg ctcaggaagt cgctcagatg
     1441 gttcgcctga aactgccggt tatcatcttc ttgatcaata actatggtta caccatcgaa
     1501 gttatgatcc atgatggtcc gtacaacaac atcaagaact gggattatgc cggtctgatg
     1561 gaagtgttca acggtaacgg tggttatgac agcggtgctg gtaaaggcct gaaggctaaa
     1621 accggtggcg aactggcaga agctatcaag gttgctctgg caaacaccga cggcccaacc
     1681 ctgatcgaat gcttcatcgg tcgtgaagac tgcactgaag aattggtcaa atggggtaag
     1741 cgcgttgctg ccgccaacag ccgtaagcct gttaacaagc tcctctaata atactagaga
     1801 aagaggagaa atactagatg tctattccag aaactcaaaa agccattatc ttctacgaat
     1861 ccaacggcaa gttggagcat aaggatatcc cagttccaaa gccaaagccc aacgaattgt
     1921 taatcaacgt caagtactct ggtgtctgcc acaccgattt gcacgcttgg catggtgact
     1981 ggccattgcc aactaagtta ccattagttg gtggtcacga aggtgccggt gtcgttgtcg
     2041 gcatgggtga aaacgttaag ggctggaaga tcggtgacta cgccggtatc aaatggttga
     2101 acggttcttg tatggcctgt gaatactgtg aattgggtaa cgaatccaac tgtcctcacg
     2161 ctgacttgtc tggttacacc cacgacggtt ctttccaaga atacgctacc gctgacgctg
     2221 ttcaagccgc tcacattcct caaggtactg acttggctga agtcgcgcca atcttgtgtg
     2281 ctggtatcac cgtatacaag gctttgaagt ctgccaactt gagagcaggc cactgggcgg
     2341 ccatttctgg tgctgctggt ggtctaggtt ctttggctgt tcaatatgct aaggcgatgg
     2401 gttacagagt cttaggtatt gatggtggtc caggaaagga agaattgttt acctcgctcg
     2461 gtggtgaagt attcatcgac ttcaccaaag agaaggacat tgttagcgca gtcgttaagg
     2521 ctaccaacgg cggtgcccac ggtatcatca atgtttccgt ttccgaagcc gctatcgaag
     2581 cttctaccag atactgtagg gcgaacggta ctgttgtctt ggttggtttg ccagccggtg
     2641 caaagtgctc ctctgatgtc ttcaaccacg ttgtcaagtc tatctccatt gtcggctctt
     2701 acgtggggaa cagagctgat accagagaag ccttagattt ctttgccaga ggtctagtca
     2761 agtctccaat aaaggtagtt ggcttatcca gtttaccaga aatttacgaa aagatggaga
     2821 agggccaaat tgctggtaga tacgttgttg acacttctaa ataataatac tagagccagg
     2881 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2941 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     3001 tata
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