BBa_K273032 1 E1A1 - T E1A1 - Ter 2009-10-17T11:00:00Z 2015-05-08T01:11:44Z Assembled by the Uppsala iGEM Team 2009 E1A1 + Terminator false false _373_ 0 5250 9 It's complicated false - false Karl Brune component2049742 1 BBa_B0012 component2049740 1 BBa_B0010 component2049739 1 BBa_K273020 annotation2049739 1 BBa_K273020 range2049739 1 1 20 annotation2049742 1 BBa_B0012 range2049742 1 117 157 annotation2049740 1 BBa_B0010 range2049740 1 29 108 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K273020 1 E1A1 E1A1 2009-10-15T11:00:00Z 2015-05-08T01:11:44Z Created with... Against target sequence ... E1A1 regulatory RNA against the Pyruvate dehydrogenase subunit one (E1), the alfa subunit of said protein (A, and number one such RNA created. Will be expressed with non free biobrick interfaces which hopefully not affect performance. false false _373_ 0 4680 9 Not in stock false - false Anders Kristoffersson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K273020_sequence 1 aaggggaaggggttttatat BBa_K273032_sequence 1 aaggggaaggggttttatattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z