BBa_K273021 1 E1A2 E1A2 2009-10-16T11:00:00Z 2015-05-08T01:11:44Z Antisense RNA function supposed to fuction through either Shine Dalgarno blocking and/or RNA elongation blocking. E1A2 antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is pyruvate dehydrogenase E1 (EC 1.2.4.1), in partuclar the alfa subunit (A). Antisense approach number 1. Will be expressed with free biobrick interfaces which hopefully not affect performance. E1A1 E1: E1 enzyme subunit of the PDC complex A: Alpha subunit of the particular enzyme subunit 1: # of antisense RNA approach false false _373_ 0 5250 9 Not in stock false false Karl Brune BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K273033 1 BBa_K273033 E1A2 - Ter 2009-10-17T11:00:00Z 2015-05-08T01:11:44Z . - false false _373_ 0 5250 9 It's complicated false - false Karl Brune component2049746 1 BBa_K273021 component2049747 1 BBa_B0010 component2049749 1 BBa_B0012 annotation2049746 1 BBa_K273021 range2049746 1 1 30 annotation2049747 1 BBa_B0010 range2049747 1 39 118 annotation2049749 1 BBa_B0012 range2049749 1 127 167 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K273021_sequence 1 ccatagtacaatttgcagtagataaagggg BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K273033_sequence 1 ccatagtacaatttgcagtagataaaggggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z